PMID: 11903629Mar 21, 2002Paper

Germline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element

Insect Molecular Biology
G L GrossmanM Q Benedict

Abstract

Germline transformation of the major African malaria vector, Anopheles gambiae, was achieved using the piggyBac transposable element marked with the enhanced green fluorescent protein (EGFP) injected into mosquito embryos. Two G1 generation male mosquitoes expressing EGFP were identified among 34 143 larvae screened. Genomic Southern data and sequencing of the piggyBac insertion boundaries showed that these two males arose from one piggyBac insertion event in the injected G0 embryos. Genetic cross data suggest that the insertion site of the element either resulted in, or is tightly linked to, a recessive lethal. This was demonstrated by a deficiency in the number of EGFP-expressing offspring from inbred crosses but expected ratios in outcrosses to non-transformed individuals and failure to establish a pure-breeding line. The insertion was weakly linked to the collarless locus on chromosome 2 and was shown by in situ hybridization to be located in division 28D of that chromosome. Particularly high levels of expression were observed uniformly in salivary glands and, in most individuals, in the anterior stomach. An improvement in the injection technique at the end of the studies resulted in increased G0 hatching, transient express...Continue Reading

References

Jul 1, 1987·The American Journal of Tropical Medicine and Hygiene·F H CollinsV Finnerty
Feb 1, 1997·Insect Molecular Biology·R W Beeman, D M Stauth
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N JasinskieneF H Collins
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·C J CoatesA A James
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·A M HandlerS H Saul
Nov 5, 1999·The American Journal of Tropical Medicine and Hygiene·M Q BenedictF H Collins
Dec 10, 1999·Nature·A J BerghammerE A Wimmer
Jan 15, 2000·Insect Molecular Biology·A M Handler, R A Harrell
Feb 15, 2000·Insect Molecular Biology·A C PinkertonP W Atkinson

❮ Previous
Next ❯

Citations

Jan 14, 2011·Parasitology Research·Kamaraju RaghavendraAditya P Dash
Jul 20, 2007·Molecular Genetics and Genomics : MGG·Christina ScaliFlaminia Catteruccia
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Alan S RobinsonPeter W Atkinson
Nov 19, 2002·International Journal for Parasitology·Luciano A MoreiraMarcelo Jacobs-Lorena
Jul 25, 2003·Veterinary Parasitology·Serap Aksoy
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Alfred M Handler
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
Jun 27, 2003·Insect Biochemistry and Molecular Biology·Jesus G ValenzuelaJosé M C Ribeiro
Aug 14, 2003·Insect Biochemistry and Molecular Biology·José M C Ribeiro
Feb 15, 2003·Trends in Parasitology·Anil GhoshMarcelo Jacobs-Lorena
Mar 20, 2003·Trends in Parasitology·Kirkwood M Land
Aug 7, 2003·Trends in Parasitology·Stefan M Kanzok, Liangbiao Zheng
Oct 9, 2002·Nature·Ennio De Gregorio, Bruno Lemaitre
Oct 26, 2005·Nature Biotechnology·Flaminia CatterucciaAndrea Crisanti
Apr 5, 2007·Nature Protocols·Neil F LoboFrank H Collins
Apr 14, 2004·Nature Reviews. Microbiology·UNKNOWN World Health Organization Special Programme for Research and Training in Tropical Diseases (WHO/TDR) 2004
Jan 25, 2003·Cellular Microbiology·George Dimopoulos
Apr 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rebeca Carballar-LejarazúAnthony A James
Aug 22, 2002·EMBO Reports·Stéphanie BlandinElena A Levashina
Aug 30, 2007·Nucleic Acids Research·Nikolai WindbichlerAndrea Crisanti
Jul 31, 2003·Nucleic Acids Research·Anthony E BrownFlaminia Catteruccia
Aug 13, 2004·Proceedings. Biological Sciences·Steven P Sinkins, H Charles J Godfray
Jan 25, 2005·Insect Molecular Biology·X NirmalaA A James
Sep 5, 2002·Annual Review of Entomology·Jaroslaw Krzywinski, Nora J Besansky
Aug 31, 2011·Journal of Tropical Medicine·Chris M CirimotichGeorge Dimopoulos
Mar 11, 2003·Annals of Tropical Medicine and Parasitology·H Townson
Mar 30, 2006·Malaria Journal·Friedrich FrischknechtRobert Menard
Dec 16, 2009·Malaria Journal·Flaminia CatterucciaErnst A Wimmer
Jun 22, 2011·Mobile DNA·Amandeep S KahlonPeter W Atkinson
Apr 1, 2009·PLoS Neglected Tropical Diseases·Barry J BeatyFotis C Kafatos
Jul 19, 2013·PloS One·Azhahianambi PalavesamDavid A O'Brochta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.