Germline transgenesis of zebrafish using the medaka Tol1 transposon system

Developmental Dynamics : an Official Publication of the American Association of Anatomists
A KogaSamuel S Chong

Abstract

Tol1 is a DNA-based transposable element first identified from an albino mutant medaka fish. It has been demonstrated to function as an efficient gene transfer vector in mammalian cells. We now demonstrate Tol1 germline transgenesis in zebrafish. A construct containing the green fluorescence protein (GFP) reporter gene inserted between the Tol1 arms was microinjected together with Tol1 transposase mRNA into fertilized eggs. Sustained GFP expression was observed in 88% of 1-month-old fish, suggesting efficient transposon integration into somatic cells. Eleven of 24 adult GFP-positive fish yielded GFP-positive progeny. Sequencing analysis of Tol1 insertion sites in GFP-positive progeny confirmed Tol1 transposition-mediated integrations into zebrafish chromosomes. We also observed functional independence of the Tol1 transposase-substrate system from that of Tol2, another medaka-derived transposon. Coupled with its previously demonstrated maximal cargo capacity of >20 kb, Tol1 could serve as a useful addition to the zebrafish genetic engineering toolbox.

References

Sep 5, 1996·Nature·A KogaH Hori
Jul 17, 2001·Genetics·E RubinA A Levy
May 25, 2002·Zoological Science·Akihiko KogaHiroshi Hori
Nov 5, 2003·Developmental Biology·Ann E DavidsonStephen C Ekker
Dec 18, 2004·Methods in Cell Biology·Spencer HermansonStephen C Ekker
Sep 5, 2006·Genetics·Alexander EmelyanovSerguei Parinov
Nov 14, 2006·PLoS Genetics·Darius BalciunasStephen C Ekker
Jun 8, 2007·Journal of Human Genetics·Akihiko KogaSatoshi Hamaguchi

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Citations

Sep 17, 2013·Molecular Genetics and Genomics : MGG·Viktorija GrajevskajaDarius Balciunas
Dec 26, 2008·Briefings in Functional Genomics & Proteomics·Jun NiStephen C Ekker
Nov 13, 2012·Nucleic Acids Research·Peng HuangBo Zhang
Dec 9, 2010·Mobile DNA·Zoltán Ivics, Zsuzsanna Izsvák
Oct 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tohei YokogawaHarold A Burgess
Jan 1, 2013·Global Cardiology Science & Practice·Kar Lai Poon, Thomas Brand
Jun 7, 2014·Genome Génome / Conseil National De Recherches Canada·Kohei WatanabeAkihiko Koga
Aug 25, 2010·Annual Review of Genetics·Hiroyuki Takeda, Atsuko Shimada
Jun 3, 2014·The Journal of Physiology·James A Shapiro
Oct 2, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Peng HuangBo Zhang
Apr 24, 2012·Trends in Biotechnology·Wiebke GarrelsWilfried A Kues
Dec 5, 2015·Frontiers in Neural Circuits·Gregory D MarquartHarold A Burgess
May 30, 2009·Nature Methods·Zoltán IvicsZsuzsanna Izsvák
Sep 17, 2014·Molecular Psychiatry·S A BergeronH A Burgess
Jul 6, 2017·Journal of Neurogenetics·Stephanie Niklaus, Stephan C F Neuhauss
Nov 2, 2016·Development·Thomas WeberReinhard W Köster
Jun 21, 2019·PLoS Biology·Abiud Cantu GutierrezJoshua D Wythe
Jun 1, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christian MosimannLeonard I Zon
Jun 7, 2021·The Journal of Biological Chemistry·Astrid BuchbergerReinhard W Köster
Jul 25, 2021·International Journal of Molecular Sciences·Mohamed A ElsaeyReinhard W Köster
Sep 17, 2021·Genome Génome / Conseil National De Recherches Canada·Sakura HayashiAkihiko Koga

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