PMID: 7581517Nov 1, 1995Paper

Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors

Transgenic Research
P ThoravalG Dambrine

Abstract

We have used vectors derived from avian leukosis viruses to transduce exogenous genes into early somatic stem cells of chicken embryos. The ecotropic helper cell line, Isolde, was used to generate stocks of NL-B vector carrying the Neo(r) selectable marker and the Escherichia coli lacZ gene. Microinjection of the NL-B vector directly beneath unincubated chicken embryo blastoderms resulted in infection of germline stem cells. One of the 16 male birds hatched (6.25%) from the injected embryos contained vector DNA sequences in its semen. Vector sequences were transmitted to G1 progeny at a frequency of 2.7%. Neo(r) and lacZ genes were transcribed in vitro in chicken embryo fibroblast cultures from transgenic embryos of the G2 progeny.

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Citations

Jan 13, 2006·Cell and Tissue Research·Paul E MozdziakJames N Petitte
Dec 14, 2002·Trends in Biotechnology·Robert Ivarie
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·James N Petitte, Paul E Mozdziak
Nov 26, 2009·Animal Biotechnology·Tomislav Modric, Ayalew Mergia
Jun 24, 2010·Theriogenology·Sang Hyun ParkJae Yong Han
Feb 6, 2008·Comparative Immunology, Microbiology and Infectious Diseases·Jae Yong Han
Jan 3, 2013·Development, Growth & Differentiation·Ken-ichi Nishijima, Shinji Iijima
Jun 29, 2004·Biochemical and Biophysical Research Communications·Mo Sun KwonTeoan Kim
Aug 7, 2004·Mechanisms of Development·J N PetitteZ Yang
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Mar 30, 2002·Nature Biotechnology·Alex J HarveyRobert Ivarie
Jul 6, 2004·Molecular Reproduction and Development·Bon Chul KooTeoan Kim
Jul 28, 2004·Molecular Reproduction and Development·Tracy AndachtRobert Ivarie
Feb 10, 2018·Journal of Animal Science and Biotechnology·Jae Yong Han, Young Hyun Park
Mar 6, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P E MozdziakJ N Petitte
Mar 9, 2021·Frontiers in Genetics·Collins N Khwatenge, Samuel N Nahashon
Aug 7, 2004·Mechanisms of Development·Helen Sang

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