Gestational exposure to valproic acid upregulates total Stat3 protein expression while downregulating phosphorylated Stat3 in CD-1 mouse embryos with neural tube defects.

Birth Defects Research
Sidra Shafique, Louise M Winn

Abstract

Valproic acid (VPA), a widely prescribed antiepileptic drug and an effective treatment for psychiatric disorders, is teratogenic causing neural tube defects (NTDs) and other defects in the exposed embryo. Signal transducer and activator of transcription 3 (Stat3) is a transcription factor that is activated via tyrosine phosphorylation. Stat3, as well as its active form (pYStat3), is expressed during neural tube closure in murine development. This study investigated the effects of in utero VPA exposure on embryonic Stat3 mRNA and protein expression during the critical period of neural tube closure in CD-1 mouse embryos. Following the exposure of CD-1 pregnant mice to the teratogenic dose of 400 mg/kg VPA or saline on gestational day (GD) 9, embryos were harvested at 1, 3, 6, or 24 hr and on GD13. Stat3 mRNA levels remained unchanged at all time points. Total Stat3 protein levels were significantly (p < .05) increased in GD9 embryos at 1 and 6 hr post-exposure and in GD13 exposed nonexencephalic and exencephalic embryo heads. In contrast, phosphorylated Stat3 levels were significantly (p < .05) downregulated in GD9 embryos at the 3 and 6 hr time points with an overall trend of downregulation in the GD10 and GD13 groups. Total and...Continue Reading

References

Feb 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S A DuncanJ E Darnell
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·K TakedaS Akira
Oct 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M H Kuo, C D Allis
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 7, 2002·Developmental Cell·Susumu YamashitaToshio Hirano
Aug 21, 2003·The Journal of Clinical Psychiatry·S Nassir GhaemiDouglas J Hsu
Feb 12, 2004·Cancer Research·Nadia GurvichPeter S Klein
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ye YanNaihe Jing
Aug 23, 2005·Toxicology and Applied Pharmacology·Jennifer E DawsonLouise M Winn
Oct 22, 2005·Lancet Neurology·Emilio Perucca
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Magdalena Götz, Wieland B Huttner
May 31, 2006·Congenital Anomalies·Rengasamy Padmanabhan
Oct 24, 2009·Nature Reviews. Cancer·Hua YuRichard Jove
Nov 17, 2009·Cell Death and Differentiation·K NihiraK Yoshida
Dec 19, 2009·Handbook of Experimental Pharmacology·Peter G WellsPatricia A Harper
Jun 22, 2010·The Journal of Biological Chemistry·Yan LiuJiayuh Lin
Aug 14, 2010·Toxicology and Applied Pharmacology·Emily W Y Tung, Louise M Winn
Mar 24, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Måns JergilMichael Stigson
Oct 11, 2011·Epilepsy Research·Linda J StephenMartin J Brodie
Feb 2, 2013·Journal of Neurology, Neurosurgery, and Psychiatry·Rebecca Louise BromleyUNKNOWN Liverpool and Manchester Neurodevelopment Group
Jun 5, 2013·Birth Defects Research. Part A, Clinical and Molecular Teratology·Demilade A AdedinsewoGodfrey P Oakley
Sep 17, 2013·Ultrasound in Medicine & Biology·Orlando AristizábalDaniel H Turnbull
Jul 30, 2014·Birth Defects Research. Part A, Clinical and Molecular Teratology·Kristin FatheRichard H Finnell
Jun 1, 2012·Journal of Developmental Origins of Health and Disease·T C HrubecS D Holladay
Jan 23, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Seulgi Hong, Mi-Ryoung Song
Jun 6, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Neil Vargesson
Mar 18, 2016·Genetics and Molecular Research : GMR·Y DengL D Zhang
Jul 17, 2016·Pediatrics and Neonatology·Hatice Mutlu-AlbayrakHüseyin Çaksen
Feb 16, 2017·Development·Evanthia NikolopoulouAndrew J Copp
Sep 25, 2017·BMJ : British Medical Journal·Jacqui Wise
Jan 7, 2018·Journal of Reproductive Immunology·Sarah A RobertsonHannah M Brown
Feb 2, 2018·PloS One·Mohammad Ali OkhovatNegin Nikouyan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Clinical Investigation
David E Levy, Chien-kuo Lee
Genesis : the Journal of Genetics and Development
Mariah G AlorroFrederic Masson
Nature Reviews. Cancer
Sarah Seton-Rogers
© 2021 Meta ULC. All rights reserved