PMID: 12771236May 29, 2003Paper

Gestational profile of matrix metalloproteinases in rat uterine artery

Molecular Human Reproduction
B A KellyL Poston

Abstract

Mechanisms underlying structural reorganization of the uterine artery in pregnancy remain largely unknown. Matrix metalloproteinases (MMPs) which are involved in degradation of vascular wall matrix are likely to play a key role. In this investigation of rat uterine artery, key MMPs and the specific tissue inhibitors of MMPs (TIMPs) together with three housekeeping genes were studied before, during and after pregnancy, using real time PCR. Data were analysed by partial least squares analysis as well as by conventional univariate methods. Each gene studied [MMP-2, MMP-3, MMP-7, MMP-9, MMP-12, MMP-13, membrane-type 1 (MT1)-MMP, TIMP-1, TIMP-2, GAPDH, cyclophilin and beta-actin] increased in late pregnancy (day 21). MMP-2, MT1MMP, MMP-3 and TIMP-1 transcripts were also elevated at day 7. TIMP-1 and MMP-3 mRNA expression returned to virgin control values in the post-partum, whereas others remained elevated or increased further (MMP-9, MMP-13). Gelatin zymography showed maximum elevation of MMP-2 at day 21. A novel 43-45 kDa gelatinolytic doublet was observed which increased in density with gestation and may represent an active MMP-2 fragment. Together, these data strongly suggest that MMPs and TIMPs are likely to play an important r...Continue Reading

Citations

Mar 16, 2012·Journal of Human Hypertension·V J KarthikeyanA D Blann
Jun 30, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lenka A VodstrcilLaura J Parry
Aug 23, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Sarah A HaleGeorge Osol
Aug 14, 2010·Obstetrics and Gynecology International·Nicole S Olgun, Sandra E Reznik
Aug 7, 2007·Biochemical Pharmacology·Joseph D Raffetto, Raouf A Khalil
Mar 17, 2009·Journal of Clinical Laboratory Analysis·Martina MontagnanaGian Cesare Guidi
Aug 21, 2008·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Arezou RezaeiAhmad Reza Dehpour
Nov 10, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Flávia Bittencourt BrasilCristiane da Fonte Ramos
May 3, 2011·American Journal of Obstetrics and Gynecology·Joris van DrongelenMarc E A Spaanderman
Apr 1, 2004·Expert Opinion on Therapeutic Patents·Nicolò Pipitone, Costantino Pitzalis
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Mar 22, 2020·International Journal of Molecular Sciences·Kathirvel Gopalakrishnan, Sathish Kumar
Apr 12, 2020·International Journal of Molecular Sciences·Maurizio Mandalà

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