Gestational trophoblastic diseases: 1. Pathophysiology of hyperglycosylated hCG

Gynecologic Oncology
Laurence A ColeErnest Kohorn

Abstract

Hyperglycosylated hCG (hCG-H) is a glycosylation variant of hCG produced by cytotrophoblast cells at implantation of pregnancy and in choriocarcinoma. We investigated the biological function of hCG-H in invasion in vitro and in vivo and the use of hCG-H antibodies in blocking tumorigenesis and cancer growth in vivo. hCG-H accounts for 43% to 100% of total hCG immunoreactivity in the culture fluid of choriocarcinoma cell lines and 100% in primary cultures of pregnancy cytotrophoblast cells. We investigated the action of hCG and hCG-H on isolated cytotrophoblast cell primary cultures and on 3 different lines of choriocarcinoma cells cultured on Matrigel basement membrane inserts (culture models for assessing tumor invasion). The addition of hCG-H to medium significantly promoted invasion of membranes with both pregnancy and cancer cell line sources, while regular hCG had no significant effect. JEG-3 human choriocarcinoma cells were transplanted subcutaneously into athymic nude mice. Tumors rapidly formed. B152, mouse monoclonal antibody against hCG-H, and non-specific mouse IgG (control) were administered twice weekly once tumors were clearly visible. While a correlation between time and growth was observed with the control group...Continue Reading

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Citations

Feb 18, 2014·Current Obstetrics and Gynecology Reports·Siew-Fei Ngu, Karen K L Chan
Jan 28, 2009·Reproductive Biology and Endocrinology : RB&E·Laurence A Cole
Aug 17, 2011·Reviews in Endocrine & Metabolic Disorders·Prajna Banerjee, Asgerally T Fazleabas
Oct 1, 2015·Cell Adhesion & Migration·Jean-Jacques Candelier
Dec 8, 2009·Gynecologic Oncology·Diljeet K Singh, John R Lurain
Aug 7, 2007·Fertility and Sterility·Yasushi SasakiLaurence A Cole
Mar 10, 2007·Placenta·L A Cole
Mar 6, 2009·American Journal of Reproductive Immunology : AJRI·Sutatip Pongcharoen, Kwansuda Supalap
Oct 29, 2015·BioMed Research International·Alan KirwanMichelle Kilcoyne
Apr 12, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Megan P T ColesonRyan L Ashley
Feb 18, 2011·American Journal of Obstetrics and Gynecology·Laurence A Cole
Dec 2, 2011·Lancet·Annie N Y Cheung, Karen K L Chan
May 25, 2010·Clinical Biochemistry·Laurence A ColeCarolyn Y Muller
Aug 24, 2016·Archives of Gynecology and Obstetrics·Giuseppe SantoroVittorio Palmara
Jun 30, 2009·Journal of Reproductive Immunology·Laurence A Cole
Apr 25, 2008·Fertility and Sterility·Laurence A ColeFrancis W Byrn
Sep 1, 2007·Current Opinion in Oncology·Sarah Ngan, Michael J Seckl
Jan 30, 2008·Nature Clinical Practice. Oncology·Michael E ColeRalph S Freedman
Apr 25, 2012·Clinical Chemistry·Anna LempiäinenUlf-Håkan Stenman
Apr 19, 2012·Clinical Obstetrics and Gynecology·Kevin Visconti, Nikki Zite
Dec 13, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah BerndtJean-Michel Foidart
Jun 30, 2012·The Journal of Clinical Endocrinology and Metabolism·John R CrochetThomas M Price
Apr 2, 2020·Frontiers in Immunology·Virginie GrideletVincent Geenen
Jan 12, 2021·Computational and Structural Biotechnology Journal·Léa V ZinsliMathias Schmelcher
Jun 29, 2021·Therapeutic Advances in Reproductive Health·Oluwafunmilayo OyatogunDanny J Schust
May 9, 2014·Journal of the American Chemical Society·Alberto Fernández-TejadaSamuel J Danishefsky
Nov 15, 2008·Gynecologic Oncology·Carolyn Y Muller, Laurence A Cole
Dec 27, 2011·Gynecologic Oncology·Laurence A Cole

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