Getting from A to B-exploring the activation motifs of the class B adhesion G protein-coupled receptor subfamily G member 4/GPR112

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Miriam C PeetersThue W Schwartz

Abstract

The adhesion G protein-coupled receptors [ADGRs/class B2 G protein-coupled receptors (GPCRs)] constitute an ancient family of GPCRs that have recently been demonstrated to play important roles in cellular and developmental processes. Here, we describe a first insight into the structure-function relationship of ADGRs using the family member ADGR subfamily G member 4 (ADGRG4)/GPR112 as a model receptor. In a bioinformatics approach, we compared conserved, functional elements of the well-characterized class A and class B1 secretin-like GPCRs with the ADGRs. We identified several potential equivalent motifs and subjected those to mutational analysis. The importance of the mutated residues was evaluated by examining their effect on the high constitutive activity of the N-terminally truncated ADGRG4/GPR112 in a 1-receptor-1-G protein Saccharomyces cerevisiae screening system and was further confirmed in a transfected mammalian human embryonic kidney 293 cell line. We evaluated the results in light of the crystal structures of the class A adenosine A2A receptor and the class B1 corticotropin-releasing factor receptor 1. ADGRG4 proved to have functionally important motifs resembling class A, class B, and combined elements, but also a u...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jul 5, 2002·Journal of Molecular Biology·Matthew P JacobsonBarry Honig
Mar 27, 2004·Science·Xianhua PiaoChristopher A Walsh
Mar 30, 2004·Proteins·Matthew P JacobsonRichard A Friesner
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Dec 29, 2006·Molecular Pharmacology·G Enrico RovatiRichard R Neubig
Jan 16, 2008·Trends in Pharmacological Sciences·Brian Kobilka, Gebhard F X Schertler
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Gerrit van MeerGerald W Feigenson
Apr 9, 2008·Endocrine, Metabolic & Immune Disorders Drug Targets·Jasmin R Dromey, Kevin D G Pfleger
Jul 3, 2008·Nature·Tony WarneGebhard F X Schertler
Oct 11, 2008·Molecular Biology and Evolution·Karl J V NordströmHelgi B Schiöth
Apr 7, 2010·Journal of Receptor and Signal Transduction Research·Daisuke OkajimaHiroshi Yokota
Jun 29, 2011·The Journal of Biological Chemistry·Kevin J PaavolaRandy A Hall
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Jul 17, 2012·Science·Wei LiuRaymond C Stevens
Dec 12, 2012·Annals of the New York Academy of Sciences·Demet AraçTobias Langenhan
Feb 15, 2013·Nature·A J VenkatakrishnanM Madan Babu
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Denise WoottenPatrick M Sexton
Jul 19, 2013·Nature·Patrick M Sexton, Denise Wootten
Jul 19, 2013·Nature·Fai Yiu SiuRaymond C Stevens
Jul 19, 2013·Nature·Kaspar HollensteinFiona H Marshall
Dec 7, 2013·Nucleic Acids Research·Vignir IsbergDavid Gloriam
Dec 30, 2014·Trends in Pharmacological Sciences·Vignir IsbergDavid E Gloriam
Feb 26, 2015·Pharmacological Reviews·Jörg HamannHelgi B Schiöth
Apr 16, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Avital Horowitz, Christopher Moraes
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Hannah M StovekenGregory G Tall

❮ Previous
Next ❯

Citations

Mar 13, 2019·Basic & Clinical Pharmacology & Toxicology·Tobias Langenhan
Oct 3, 2017·Annual Review of Pharmacology and Toxicology·Ryan H Purcell, Randy A Hall
Jun 15, 2017·Functional & Integrative Genomics·Kai TianQing Zhu
Apr 20, 2020·Cellular Signalling·Robin L Maser, James P Calvet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Simone PrömelTobias Langenhan
Trends in Pharmacological Sciences
Kaspar HollensteinRaymond C Stevens
© 2021 Meta ULC. All rights reserved