GFAP promoter directs lacZ expression specifically in a rat hepatic stellate cell line

World Journal of Gastroenterology : WJG
Gunter MaubachLang Zhuo

Abstract

The GFAP was traditionally considered to be a biomarker for neural glia (mainly astrocytes and non-myelinating Schwann cells). Genetically, a 2.2-kb human GFAP promoter has been successfully used to target astrocytes in vitro and in vivo. More recently, GFAP was also established as one of the several makers for identifying hepatic stellate cells (HSC). In this project, possible application of the same 2.2-kb human GFAP promoter for targeting HSC was investigated. The GFAP-lacZ transgene was transfected into various cell lines (HSC, hepatocyte, and other non-HSC cell types). The transgene expression specificity was determined by X-gal staining of the beta-galactosidase activity. And the responsiveness of the transgene was tested with a typical pro-fibrotic cytokine TGF-beta1. The expression of endogenous GFAP gene was assessed by real-time RT-PCR, providing a reference for the transgene expression. The results demonstrated for the first time that the 2.2 kb hGFAP promoter was not only capable of directing HSC-specific expression, but also responding to a known pro-fibrogenic cytokine TGF-beta1 by upregulation in a dose- and time-dependent manner, similar to the endogenous GFAP. In conclusion, these findings suggested novel utili...Continue Reading

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Citations

Feb 25, 2011·Pharmaceutical Research·Ningning Yang, Ram I Mahato
May 7, 2013·Parasites & Vectors·Hasan Tarık AtmacaOguz Kul
Jan 19, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Shao-Long ChenYong-Ping Chen
Mar 5, 2016·Frontiers in Pharmacology·Hans-Theo SchonRalf Weiskirchen
Apr 13, 2018·International Journal of Molecular Sciences·Wen-Pu ShiHuijie Bian
Dec 13, 2018·Oxidative Medicine and Cellular Longevity·Hannah K DrescherDaniela C Kroy
Feb 4, 2011·Pediatric Surgery International·Ken-ichi MurakamiHideo Takamatsu

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