GFP-expressing S100β-positive cells of the rat anterior pituitary differentiate into hormone-producing cells

Cell and Tissue Research
Masashi HiguchiYukio Kato

Abstract

Some non-endocrine cells in the pituitary anterior lobe are responsible for providing stem/progenitor cells to maintain hormone-producing cells. In particular, cells expressing S100β protein, a calcium-binding protein, have been hypothesized to be a pituitary cell resource. Accumulating data have revealed that S100β-positive cells comprise heterogeneous populations and some of them certainly show stem/progenitor characteristics in vivo. Hence, we examine whether S100β-positive cells have the capacity to differentiate into endocrine cells, by means of in vivo and in vitro experiments on transgenic rats expressing enhanced green fluorescent protein (EGFP) under the control of the S100β promoter. Immunohistochemistry of the pituitary confirmed that some S100β-positive cells expressed SOX2 (SRY [sex-determining region Y]-box 2) and had proliferative activity. Dispersed anterior lobe cells were observed by time-lapse microscopy, followed by immunostaining for hormone and pituitary-transcription-factor1 (PIT1). First, the dispersed anterior lobe cells were immunostained by an antibody against SOX2. S100β-protein co-localizes with SOX2 (about 89 %). Although 44 of 134 S100β-positive cells traced were proliferative but negative to any ...Continue Reading

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Citations

Jan 14, 2016·International Journal of Molecular Sciences·Saishu YoshidaYukio Kato
May 15, 2018·Biology of Reproduction·Whitney Edwards, L T Raetzman
Oct 18, 2019·Frontiers in Endocrinology·Patrick A FletcherStanko S Stojilkovic
Mar 20, 2020·International Journal of Molecular Sciences·Branka Šošić-JurjevićVerica Milošević
Dec 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Charlotte SteenblockStefan R Bornstein
Nov 23, 2020·Histochemistry and Cell Biology·Kotaro HoriguchiShunji Ohsako

Related Concepts

S100b protein, rat
Cell Density
Cell Differentiation Process
Cell Division Phases
Immunocytochemistry
Pituitary Gland, Anterior
Pituitary Hormones, Anterior
Trypure
Ki-67 Antigen
Green Fluorescent Proteins

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