GFP reporter mice for the retinoblastoma-related cell cycle regulator p107.

Cell Cycle
Deborah L BurkhartJulien Sage

Abstract

The RB tumor suppressor gene is mutated in a broad range of human cancers, including pediatric retinoblastoma. Strikingly, however, Rb mutant mice develop tumors of the pituitary and thyroid glands, but not retinoblastoma. Mouse genetics experiments have demonstrated that p107, a protein related to pRB, is capable of preventing retinoblastoma, but not pituitary tumors, in Rb-deficient mice. Evidence suggests that the basis for this compensatory function of p107 is increased transcription of the p107 gene in response to Rb inactivation. To begin to address the context-dependency of this compensatory role of p107 and to follow p107 expression in vivo, we have generated transgenic mice carrying an enhanced GFP (eGFP) reporter inserted into a bacterial artificial chromosome (BAC) containing the mouse p107 gene. Expression of the eGFP transgene parallels that of p107 in these transgenic mice and identifies cells with a broad range of expression level for p107, even within particular organs or tissues. We also show that loss of Rb results in the upregulation of p107 transcription in specific cell populations in vivo, including subpopulations of hematopoietic cells. Thus, p107 BAC-eGFP transgenic mice serve as a useful tool to identif...Continue Reading

Citations

Feb 4, 2011·Molecular Biology of the Cell·Ryan J BourgoErik S Knudsen
Apr 3, 2010·Cell Division·Stacey E Wirt, Julien Sage
Aug 12, 2017·Critical Reviews in Biochemistry and Molecular Biology·Martin Fischer, Gerd A Müller
Jan 27, 2010·Molecular and Cellular Biology·Deborah L BurkhartJulien Sage
Aug 18, 2009·Current Opinion in Ophthalmology

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