GFP tagging sheds light on protein translocation: implications for key methods in cell biology.

Cellular and Molecular Life Sciences : CMLS
Marcel Deponte

Abstract

Green fluorescent protein (GFP) is a powerful tool for studying gene expression, protein localization, protein-protein interactions, calcium concentrations, and redox potentials owing to its intrinsic fluorescence. However, GFP not only contains a chromophore but is also tightly folded in a temperature-dependent manner. The latter property of GFP has recently been exploited (1) to characterize the translocase of the outer mitochondrial membrane and (2) to discriminate between protein transport across and into biomembranes in vivo. I therefore suggest that GFP could be a valuable tool for the general analysis of protein transport machineries and pathways in a variety of organisms. Moreover, results from such studies could be important for the interpretation and optimization of classical experiments using GFP tagging.

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Citations

Oct 10, 2012·Molecular and Biochemical Parasitology·Marcel DeponteJude M Przyborski
Dec 4, 2012·Biochimica Et Biophysica Acta·Philip G Board, Deepthi Menon
Feb 12, 2013·Biochimica Et Biophysica Acta·Bruno MantaRafael Radi
Jun 12, 2019·PLoS Computational Biology·Mahua Roy, Stacey D Finley
Aug 24, 2016·PloS One·Christie A BaderDouglas A Brooks
Aug 21, 2013·Journal of Biomolecular Screening·Simone C AlbrechtAndreas J Meyer
Nov 10, 2020·Molecular and Biochemical Parasitology·Gino L TurraMarcel Deponte

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