GH4 pituitary cell variants selected as nonresponsive to thyrotropin-releasing hormone-enhanced substratum adhesion are nonresponsive to epidermal growth factor: evidence for a common signaling defect.

Journal of Cellular Physiology
J S Ramsdell, A H Tashjian

Abstract

Thyrotropin-releasing hormone (TRH) and epidermal growth factor both enhance prolactin synthesis and substrate adhesion (a morphological change called stretching) of GH4 rat pituitary cells. We have examined TRH- and EGF-induced cell stretching using genetic and pharmacologic approaches. We selected and isolated a series of GH4 cell variants nonresponsive to TRH-induced cell stretching (str-). This selection yielded several variants that were nonresponsive to both TRH- and EGF-induced stretching but were still responsive to stretching induced by several other agents (tetradecanoylphorbol acetate [TPA], butyrate, and Neplanocin A). One of the str- variants (a14) was examined in detail. TRH, EGF, and TPA each enhanced prolactin synthesis in a14 cells, indicating that the a14 variant contained functional receptor binding sites for all 3 ligands as well as the capacity to generate those intracellular signals required for enhanced prolactin synthesis. Because the str- variants were isolated without selective pressure for EGF-induced stretching and because the possibility of more than one selectable mutation in all the variants is unlikely, we suggest that TRH and EGF share a common mechanism to induce cell stretching. We next examin...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·A H Tashjian
Oct 1, 1985·Experimental Cell Research·E G HaymanE Ruoslahti
Nov 1, 1988·Molecular Endocrinology·A E Jackson, C Bancroft
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Jan 1, 1988·Annual Review of Biochemistry·E Ruoslahti
Dec 1, 1985·Molecular and Cellular Endocrinology·J S Ramsdell, A H Tashjian
Jan 1, 1987·Kardiologiia·Iu B BelousovE P Panchenko
Jan 1, 1983·Annual Review of Biochemistry·K M Yamada

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Citations

Mar 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J S Ramsdell
Nov 16, 2004·Molecular and Cellular Endocrinology·Guck T OoiRuth M Escalona

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