GhCPK33 Negatively Regulates Defense against Verticillium dahliae by Phosphorylating GhOPR3

Plant Physiology
Qin HuXianlong Zhang

Abstract

Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is a destructive vascular disease in plants. Approximately 200 dicotyledonous plant species in temperate and subtropical regions are susceptible to this notorious pathogen. Previous studies showed that jasmonic acid (JA) plays a crucial role in plant-V. dahliae interactions. V. dahliae infection generally induces significant JA accumulation in local and distal tissues of the plant. Although JA biosynthesis and the associated enzymes have been studied intensively, the precise mechanism regulating JA biosynthesis upon V. dahliae infection remains unknown. Here, we identified the calcium-dependent protein kinase GhCPK33 from upland cotton (Gossypium hirsutum) as a negative regulator of resistance to V. dahliae that directly manipulates JA biosynthesis. Knockdown of GhCPK33 by Agrobacterium tumefaciens-mediated virus-induced gene silencing constitutively activated JA biosynthesis and JA-mediated defense responses and enhanced resistance to Vdahliae Further analysis revealed that GhCPK33 interacts with 12-oxophytodienoate reductase3 (GhOPR3) in peroxisomes. GhCPK33 phosphorylates GhOPR3 at threonine-246, leading to decreased stability of GhOPR3, which consequen...Continue Reading

Citations

Aug 24, 2019·The New Phytologist·Ronghui PanJianping Hu
Feb 13, 2020·International Journal of Molecular Sciences·Ranran SongXingyong Yang
Dec 5, 2020·International Journal of Biological Macromolecules·Hongjie FengHeqin Zhu
Dec 1, 2020·Frontiers in Plant Science·Nikhilesh DharSteven J Klosterman
Jan 26, 2020·Trends in Plant Science·Zuoren YangFuguang Li
Mar 20, 2021·Journal of Agricultural and Food Chemistry·Boqing ZhaoFang Yuan

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