Ghrelin and truncated ghrelin variant plasmid vectors administration into skeletal muscle augments long-term growth in rats

Domestic Animal Endocrinology
Q F XieN Li

Abstract

Ghrelin is an acylated peptide recently identified as an endogenous ligand for the growth hormone (GH) secretagogues (GHSs) receptor (GHS-R) and is involved in a novel system for regulating GH release. To study the biological activities of ghrelin using plasmid vector administration, we constructed myogenic expression vectors containing the full length cDNA of swine ghrelin-28 (pGEM-wt-sGhln) and truncated variant (pGEM-tmt-sGhln) consisting of the first seven residues of ghrelin (including Ser3 substituted with Trp3) with addition of a basic amino acid, Lys (K) at the C-terminus. After intramuscular injection of pGEM-wt-sGhln and pGEM-tmt-sGhln, RT-PCR analysis demonstrated that the ectopic expressions of ghrelin and its variant were observed 30 days post-injection. The level of GH increased in rat serum, and was significantly higher than that of the control group 20 days post-injection with pGEM-tmt-sGhln (P < 0.05). Administration of 150 microg of pGEM-wt-sGhln and pGEM-tmt-sGhln enhanced growth in rats over 30 days and great stimulatory responses were observed at day 10 and 20 post-injection respectively, whose body weight gains were on average 15% (P < 0.05) and 21% P < 0.033 significantly heavier than controls. These resu...Continue Reading

References

Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S K TripathyJ M Leiden
Jan 1, 1996·Hormone Research·M F ScanlonC Dieguez
Jul 25, 2000·Endocrine·A L Carrel, D B Allen
Apr 12, 2001·The Journal of Clinical Endocrinology and Metabolism·C Y Bowers
Jun 16, 2001·Hormone Research·M PomboC Dieguez
Sep 11, 2001·Biochemical and Biophysical Research Communications·M MatsumotoK Kangawa
Jul 12, 2002·Current Gene Therapy·Delphine Lechardeur, Gergely L Lukacs
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruxandra Draghia-AkliMarta L Fiorotto

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Citations

Aug 28, 2010·International Journal of Peptides·Johannes D Veldhuis, Cyril Y Bowers
Jul 23, 2011·Journal of Animal Physiology and Animal Nutrition·D Montoya-FloresJ L Romano-Muñoz
Aug 5, 2006·The Journal of Pediatrics·Christine R Erdie-LalenaDavid E Cummings
Jul 25, 2006·Physiology & Behavior·David E Cummings
Jun 2, 2005·Domestic Animal Endocrinology·L L AndersonA Glavaski-Joksimovic
May 24, 2015·General and Comparative Endocrinology·María José MazónAna Gómez
Feb 1, 2005·Current Opinion in Endocrinology, Diabetes, and Obesity·David E CummingsKaren E Foster-Schubert

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