Ghrelin Stimulates Endothelial Cells Angiogenesis through Extracellular Regulated Protein Kinases (ERK) Signaling Pathway

International Journal of Molecular Sciences
Jun WangWeizhen Zhang

Abstract

Adipose tissue is hyper-vascularized. Vessels in adipose tissue not only supply nutrients and oxygen to nourish adipocytes, but also provide cytokines that regulate mass and function of adipose tissue. Understanding the fundamental mechanisms how vessels modulate adipocyte functions would provide new therapeutic options for treatment of metabolic disease and obesity. In recent years, researches about ghrelin are focused on glucose and lipid metabolism, but its effect on vascular function remains uncharacterized. In the present study, ghrelin receptor gene deletion mice (Ghsr-/- mice) were used to study ghrelin-regulated vascular metabolism in white adipose tissue. Ghsr-/- mice demonstrated lower food intake, lower body weight, and resistance to high-fat diet-induced obesity. The number of vessels in white adipose tissue was decreased in Ghsr-/- mice when compared with wild type mice fed with high-fat diet. To further define ghrelin effects in vitro, we used endothelial progenitor cells from wild type and Ghsr-/- mice as well as human umbilical vein endothelial cells in our experiments. We found that ghrelin stimulated endothelial cells angiogenesis and migration through the MEK-ERK signaling pathway. [d-Lys3]-GHRP-6 and PD98059...Continue Reading

References

Jun 7, 2002·The Journal of Clinical Endocrinology and Metabolism·Sharmilee GnanapavanMárta Korbonits
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maria A RupnickM Judah Folkman
May 25, 2004·Circulation Research·Ebba BråkenhielmYihai Cao
Jul 24, 2004·The Journal of Physiology·Weizhen ZhangMichael W Mulholland
Dec 3, 2005·The Journal of Clinical Investigation·Jeffrey M ZigmanJoel K Elmquist
Dec 16, 2005·Nature·Ray F Gariano, Thomas W Gardner
Aug 2, 2006·EMBO Reports·Satoko Nishimoto, Eisuke Nishida
Nov 3, 2006·Nature Reviews. Drug Discovery·Dunstan Cooke, Steve Bloom
Dec 5, 2006·The Journal of Clinical Investigation·Joseph T CrossnoDwight J Klemm
Dec 23, 2006·Biochemical and Biophysical Research Communications·Aihua LiAndrzej S Tarnawski
Apr 21, 2007·Journal of Molecular Endocrinology·Yoo Mee KimSung-Kil Lim
May 3, 2007·American Journal of Physiology. Endocrinology and Metabolism·Jan NedergaardBarbara Cannon
Sep 6, 2007·The Journal of Clinical Investigation·Yihai Cao
Nov 17, 2007·Cardiovascular Research·H Roger Lijnen
Sep 20, 2008·Science·Wei TangJonathan M Graff
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Jan 20, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Dennis Bruemmer
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kai SunPhilipp E Scherer
Apr 19, 2013·Thrombosis and Haemostasis·Amal Y LemoineEtienne Larger
Apr 23, 2013·Nature Medicine·Martin E LidellSven Enerbäck
Aug 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ziru LiWeizhen Zhang
Jun 23, 2015·Journal of Receptor and Signal Transduction Research·Yu SunHua-Fu Zhou
Sep 6, 2017·Endocrine Development·Reena Perchard, Peter E Clayton
Dec 21, 2017·PloS One·Jorge Federico ElgartUNKNOWN QUALIDIAB Net study group

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Citations

May 18, 2019·Canadian Journal of Physiology and Pharmacology·Mami NodaTakafumi Sakai
Jan 12, 2020·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hiroko MutaAkiyoshi Kakita
Aug 21, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Daniela Rosendo-Silva, Paulo Matafome
May 28, 2020·International Journal of Molecular Sciences·Mario AbateRosanna Martinelli
Oct 8, 2020·Heart Failure Reviews·Shreyasi Gupta, Arkadeep Mitra
Nov 5, 2020·The Journal of Obstetrics and Gynaecology Research·Xiaoqin WangHe Fei

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Methods Mentioned

BETA
flow cytometry
density-gradient centrifugation
Polymerase Chain Reaction
Fluorescence-activated cell sorting
FACS
Assay

Software Mentioned

Cell Quest

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