GhWRKY68 reduces resistance to salt and drought in transgenic Nicotiana benthamiana

PloS One
Haihong JiaXingqi Guo

Abstract

The WRKY transcription factors modulate numerous physiological processes, including plant growth, development and responses to various environmental stresses. Currently, our understanding of the functions of the majority of the WRKY family members and their possible roles in signalling crosstalk is limited. In particular, very few WRKYs have been identified and characterised from an economically important crop, cotton. In this study, we characterised a novel group IIc WRKY gene, GhWRKY68, which is induced by different abiotic stresses and multiple defence-related signalling molecules. The β-glucuronidase activity driven by the GhWRKY68 promoter was enhanced after exposure to drought, salt, abscisic acid (ABA) and H2O2. The overexpression of GhWRKY68 in Nicotiana benthamiana reduced resistance to drought and salt and affected several physiological indices. GhWRKY68 may mediate salt and drought responses by modulating ABA content and enhancing the transcript levels of ABA-responsive genes. GhWRKY68-overexpressing plants exhibited reduced tolerance to oxidative stress after drought and salt stress treatments, which correlated with the accumulation of reactive oxygen species (ROS), reduced enzyme activities, elevated malondialdehyd...Continue Reading

References

Dec 1, 1996·The Plant Journal : for Cell and Molecular Biology·T UraoK Shinozaki
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·J KurepaD Inzé
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·Y YangM Qi
Nov 13, 2001·The Journal of Biological Chemistry·Srinivas S L GampalaChristopher D Rock
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 5, 2002·The Plant Cell·Ruth R FinkelsteinChristopher D Rock
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Kazuya YoshimuraShigeru Shigeoka
Sep 1, 2004·Current Opinion in Plant Biology·Bekir Ulker, Imre E Somssich
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Mar 1, 2005·FEBS Letters·Chan Young ParkMoo Je Cho
Nov 1, 2006·Journal of Experimental Botany·Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
Jul 24, 2007·Current Opinion in Plant Biology·Thomas Eulgem, Imre E Somssich
Jul 25, 2009·The Plant Journal : for Cell and Molecular Biology·Julia SantiagoPedro L Rodriguez
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Nov 20, 2009·Plant & Cell Physiology·Jed A ChristiansonIain W Wilson
Nov 20, 2009·Nature·Hiroaki FujiiJian-Kang Zhu
Mar 3, 2010·Annual Review of Plant Biology·Sean R CutlerSuzanne R Abrams
Mar 23, 2010·Trends in Plant Science·Paul J RushtonQingxi J Shen
May 22, 2010·The Plant Journal : for Cell and Molecular Biology·Xiaozhi RenZhizhong Gong
May 25, 2010·Trends in Plant Science·Agepati S RaghavendraErwin Grill
Jun 24, 2011·Plant Biotechnology Journal·Deena L RushtonPaul J Rushton
Oct 4, 2011·Biochimica Et Biophysica Acta·Ligang ChenDiqiu Yu
Oct 4, 2011·Environmental Science and Pollution Research International·Xiao ShuWeiBo Wang
Jan 24, 2012·Journal of Experimental Botany·Sébastien BesseauE Tapio Palva

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Citations

Dec 21, 2016·Journal of Integrative Plant Biology·Jingjing JiangJianhua Zhang
Apr 6, 2018·The New Phytologist·Angelika Czedik-EysenbergArmin Djamei
Sep 12, 2018·International Journal of Molecular Sciences·Chang-Tao WangJin-Dong Fu
Jun 3, 2018·International Journal of Molecular Sciences·Sardar-Ali KhanHong-Ju Yin
Apr 4, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Manu PriyaHarsh Nayyar
Feb 26, 2020·Hereditas·Haiji SunXiaoli Ma
Jan 12, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·A M AbdelmoghnyV N Waghmare

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Datasets Mentioned

BETA
KJ551845

Methods Mentioned

BETA
transgenic
PCR
confocal microscopy
one-hybrid
environmental stresses
MDA
salt treatment
salt stress
ELISA
Protein Assay

Software Mentioned

MEGA
PLACE
Clustal W
CELLO
CARE
Statistical Analysis System ( SAS )
PLoc
Nuc
SAS
Plant

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