G(i)-coupled GPCR signaling controls the formation and organization of human pluripotent colonies.

PloS One
Kenta NakamuraBruce R Conklin

Abstract

Reprogramming adult human somatic cells to create human induced pluripotent stem (hiPS) cell colonies involves a dramatic morphological and organizational transition. These colonies are morphologically indistinguishable from those of pluripotent human embryonic stem (hES) cells. G protein-coupled receptors (GPCRs) are required in diverse developmental processes, but their role in pluripotent colony morphology and organization is unknown. We tested the hypothesis that G(i)-coupled GPCR signaling contributes to the characteristic morphology and organization of human pluripotent colonies. Specific and irreversible inhibition of G(i)-coupled GPCR signaling by pertussis toxin markedly altered pluripotent colony morphology. Wild-type hES and hiPS cells formed monolayer colonies, but colonies treated with pertussis toxin retracted inward, adopting a dense, multi-layered conformation. The treated colonies were unable to reform after a scratch wound insult, whereas control colonies healed completely within 48 h. In contrast, activation of an alternative GPCR pathway, G(s)-coupled signaling, with cholera toxin did not affect colony morphology or the healing response. Pertussis toxin did not alter the proliferation, apoptosis or pluripote...Continue Reading

References

Jun 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H R Kaslow, D L Burns
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Katada, M Ui
Oct 20, 1995·The Journal of Biological Chemistry·A M BuhlG L Johnson
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·P CowardB R Conklin
Nov 6, 1998·Science·J A ThomsonJ M Jones
Jun 2, 2000·Biochemical and Biophysical Research Communications·G BoguslawskiD English
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Jun 19, 2002·The Journal of Biological Chemistry·William J DeeryRichard H Gomer
Sep 7, 2002·Antioxidants & Redox Signaling·Izumi NakashimaYoshiyuki Kawamoto
May 29, 2003·The Journal of Biological Chemistry·Stephan VogtStefan Offermanns
Jan 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yumei XiongMasashi Yanagisawa
Jun 17, 2005·Annals of the New York Academy of Sciences·Gabriele SeitzRobert Möhle
Sep 27, 2005·Physiological Reviews·Nina Wettschureck, Stefan Offermanns
Oct 19, 2005·Nature Reviews. Molecular Cell Biology·Craig C Malbon
Mar 6, 2007·Developmental Cell·Ian C ScottDidier Y R Stainier
May 8, 2007·Current Biology : CB·Deenadayalan BakthavatsalamAngelika A Noegel
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jun 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Caroline R KempLuc Leyns
Nov 10, 2007·The Journal of Clinical Investigation·Jean B RegardShaun R Coughlin
Nov 22, 2007·Science·Junying YuJames A Thomson
Dec 25, 2007·Molecular Reproduction and Development·Ruchi BajpaiAlexey V Terskikh
Mar 29, 2008·Stem Cells·Timothy J NelsonAndre Terzic
Sep 13, 2008·Cell Stem Cell·Dirk HockemeyerRudolf Jaenisch
Dec 2, 2008·Cell Stem Cell·Nimet Maherali, Konrad Hochedlinger
Mar 3, 2009·Cellular Signalling·Mathieu Cotton, Audrey Claing
Mar 7, 2009·Cell Stem Cell·George Q DaleyShinya Yamanaka
Nov 7, 2009·PLoS Computational Biology·Nathan SalomonisBruce R Conklin

❮ Previous
Next ❯

Citations

Jan 16, 2014·Stem Cell Research & Therapy·Kaori MisunoShen Hu
Jul 14, 2010·Stem Cell Reviews and Reports·Nao R KobayashiAlice Pébay
Feb 11, 2011·Journal of Molecular Cell Biology·Laura CasalinoEduardo Jorge Patriarca
Jul 10, 2010·Disease Models & Mechanisms·Alethia VillasenorThomas M Wilkie
Dec 24, 2015·Frontiers in Cell and Developmental Biology·Nazanin F DolatshadGabor Földes
May 18, 2016·International Journal of Molecular Sciences·Jennifer R Lynch, Jenny Yingzi Wang
Jul 11, 2014·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Toshiaki Ishizuka, Yasuhiro Watanabe

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
PCR
flow cytometery
flow cytometry
genetic modification
scraping

Software Mentioned

Affymetrix
AltAnalyze
Photoshop
ImageJ
AxioVision
ModFit
Adobe Premiere CS3
FlowJo
Adobe Photoshop
MetaMorph

Related Concepts

Related Feeds

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.