PMID: 2510151Oct 1, 1989Paper

Gi2 mediates alpha 2-adrenergic inhibition of adenylyl cyclase in platelet membranes: in situ identification with G alpha C-terminal antibodies

Proceedings of the National Academy of Sciences of the United States of America
W F SimondsA Spiegel

Abstract

A panel of antibodies to synthetic decapeptides corresponding to the C termini of guanine nucleotide-binding regulatory protein (G protein) alpha subunits has been generated in rabbits. The specificity of each antibody was assessed by ELISA for peptide binding and by immunoblotting for binding to defined, recombinant G alpha subunits expressed in Escherichia coli. Immunoblotting of human platelet membranes with these antibodies identified a variety of endogenous G proteins including Gs (stimulatory), Gi2 (inhibitory), Gi3, and Gx(z) (unknown function). Pretreatment of platelet membranes with C-terminal antibodies reactive with Gi2, but not with antibodies to Gi3 or Gx(z), blocked alpha 2-adrenergic inhibition of adenylyl cyclase. This identifies Gi2 as the dominant mediator of cyclase inhibition in this pathway. This approach may provide a general means of identifying relevant functional interactions of G proteins with receptors and effectors in situ.

References

Jan 1, 1978·FEBS Letters·K H JakobsG Schultz
Jan 1, 1987·Molecular and Cellular Endocrinology·A M Spiegel
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M MatsuokaY Kaziro
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P BrayM Nirenberg
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P GierschikA Spiegel
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H K FongM I Simon
Jul 12, 1988·Biochemistry·M A Lochrie, M I Simon
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell

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Citations

Dec 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H M KochS Hough
Aug 1, 1993·Journal of Cellular Biochemistry·N McFarlane-AndersonN Bégin-Heick
Mar 1, 1997·Movement Disorders : Official Journal of the Movement Disorder Society·S AvissarG Schreiber
Jan 1, 1994·Protein Science : a Publication of the Protein Society·E J Neer
Apr 1, 1993·Naunyn-Schmiedeberg's Archives of Pharmacology·S BarrettJ S Karliner
Nov 1, 1993·Molecular and Cellular Biochemistry·J L MeinkothJ R Feramisco
Jan 1, 1995·Journal of Neural Transmission. General Section·M SalkovićZ Lacković
Nov 1, 1995·Journal of Clinical Immunology·U RudolphL Birnbaumer
Dec 20, 2007·Archives of Pharmacal Research·Sung Hyo KimUy Dong Sohn
Apr 24, 2010·Cardiovascular Toxicology·James H Zavecz, Harold D Battarbee
Sep 15, 1994·Biochemical Pharmacology·G MilliganC M Brown
Oct 1, 1993·Clinical Biochemistry·L S Weinstein, A Shenker
Jan 1, 1992·Life Sciences·J K HsiaoW Z Potter
Jan 1, 1992·Life Sciences·K N BitarG M Omann
Jan 1, 1994·Life Sciences·C W EmalaM A Levine
Jan 1, 1991·Pharmacology & Therapeutics·A Levitzki, A Bar-Sinai
Jul 1, 1994·Journal of Affective Disorders·Y OdagakiI Yamashita
Aug 19, 1991·Brain Research. Developmental Brain Research·L T YoungS J Kish
Jun 1, 1991·Trends in Pharmacological Sciences·G Milligan, A Green
Mar 7, 1995·Regulatory Peptides·M C Olianas, P Onali
Aug 1, 1995·Brain Research. Molecular Brain Research·D HervéM Lévi-Strauss
Aug 1, 1991·Molecular and Cellular Endocrinology·C BouvierR Collu
Jun 1, 1991·Molecular and Cellular Endocrinology·C BouvierR Collu
Apr 1, 1991·Molecular and Cellular Endocrinology·E J PaulssenJ O Gordeladze
Jun 1, 1993·Molecular and Cellular Endocrinology·H ForgetR Collu
Mar 1, 1994·Molecular and Cellular Endocrinology·M CormontY Le Marchand-Brustel
Dec 8, 1995·Neuroscience Letters·K MizutaM Tachibana

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