Gi3 mediates somatostatin-induced activation of an inwardly rectifying K+ current in human growth hormone-secreting adenoma cells

Endocrinology
K TakanoT Fujita

Abstract

SRIF activates an inwardly rectifying K+ current in human GH-secreting adenoma cells. Activation of this K+ current induces hyperpolarization of the membrane and abolishment of action potential firing. This mechanism is an essential mechanism for SRIF-induced decrease in intracellular Ca2+ concentration and inhibition of GH secretion. The activation of the inwardly rectifying K+ current is mediated by a pertussis toxin-sensitive G protein. In this article, the expression of the pertussis toxin-sensitive G protein alpha-subunits in the human GH-secreting adenoma cells were analyzed by RT-PCR, and the G protein transducing the SRIF-induced activation of this inwardly rectifying K+ current was investigated. RT-PCR of the messenger RNA from two human GH-secreting adenomas revealed that all G alpha(i1), G alpha(i2), G alpha(i3), and G alpha(o) were expressed in these adenomas. Primary cultured cells from these two adenoma cells were investigated under the voltage clamp of the whole-cell mode. Specific antibodies against the carboxyl terminus of G protein alpha-subunits were microinjected into the cells. Microinjection of antibody against the carboxyl terminal sequence of G alpha(i3) attenuated the SRIF-induced activation of the inwa...Continue Reading

References

Jan 1, 1992·Progress in Neurobiology·M Inoue, M Yoshii
Sep 1, 1992·Progress in Neurobiology·Y KurachiT Nakajima
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T TsukamotoY Kaziro
Jan 1, 1990·Annual Review of Physiology·D A Brown
Sep 1, 1990·Metabolism: Clinical and Experimental·A YataniA M Brown
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N YamashitaE Ogata
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N YamashitaE Ogata
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jun 1, 1995·Current Opinion in Neurobiology·C A DoupnikH A Lester

❮ Previous
Next ❯

Citations

Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Jan 18, 2006·Endocrine Journal·Nako Kimata-HayashiToshiro Fujita
May 12, 2007·Journal of Neurophysiology·Krasimira Tsaneva-AtanasovaStanko S Stojilkovic
Jun 27, 2012·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Koji Takano
Aug 6, 1999·Frontiers in Neuroendocrinology·Y C Patel
May 11, 2001·Neuropeptides·Z Csaba, P Dournaud
Mar 8, 2002·Cellular Signalling·Paul R Albert, Liliane Robillard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.