Giant lipid vesicles under electric field pulses assessed by non invasive imaging

Bioelectrochemistry
Chloé MauroyMarie-Pierre Rols

Abstract

We present experimental results regarding the effects of electric pulses on giant unilamellar vesicles (GUVs). We have used phase contrast and coherent anti-Stokes Raman scattering (CARS) microscopy as relevant optical approaches to gain insight into membrane changes under electropermeabilization. No addition of exogenous molecules (lipid analogue, fluorescent dye) was needed. Therefore, experiments were performed on pure lipid systems avoiding possible artefacts linked to their use. Structural membrane changes were assessed by loss of contrast inside the GUVs due to sucrose and glucose mixing. Our observations, performed at the single vesicle level, indicate these changes are under the control of the number of pulses and field intensity. Larger number of pulses enhances membrane alterations. A threshold value of the field intensity must be applied to allow exchange of molecules between GUVs and the external medium. This threshold depends on the size of the vesicles, the larger GUVs being affected at lower electric field strengths than the smaller ones. Our experimental data are well described by a simple model in which molecule entry is driven by direct exchange. The CARS microscopic study of the effect of pulse duration confi...Continue Reading

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Citations

Jul 12, 2014·Bioelectrochemistry·Feriel Siham HamdiBruno Le Pioufle
May 12, 2015·Advanced Drug Delivery Reviews·Martin Josef Winterhalder, Andreas Zumbusch
Dec 30, 2014·Colloids and Surfaces. B, Biointerfaces·Nily Dan
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Feb 5, 2019·Soft Matter·Ankush Pal, D V Khakhar
Jul 22, 2019·Bioscience Reports·Ismail DahmaniSalvatore Chiantia
Feb 24, 2017·Biotechnology for Biofuels·Alexis GuionetHamid Hosseini
Jul 25, 2017·The Journal of Membrane Biology·Tina Batista NapotnikDamijan Miklavčič
Jun 1, 2019·Scientific Reports·Dayinta L PerrierPouyan E Boukany
Jul 25, 2019·Physical Review. E·Glauber T SilvaBruce W Drinkwater
Mar 21, 2018·Scientific Reports·Dayinta L PerrierPouyan E Boukany
Oct 24, 2020·Biophysical Journal·Esin B SözerJoshua Zimmerberg

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