Giant magnetoresistive-based biosensing probe station system for multiplex protein assays

Biosensors & Bioelectronics
Yi WangJian-Ping Wang

Abstract

In this study, a sensitive immune-biosensing system capable of multiplexed, real-time electrical readout was developed based on giant magnetoresistive (GMR) sensor array to detect a panel of protein biomarkers simultaneously. PAPP-A, PCSK9, and ST2 have been regarded as promising candidate biomarkers for cardiovascular diseases. Early detection of multiple biomarkers for a disease could enable accurate prediction of a disease risk. 64 nano-size GMR sensors were assembled onto one 16mm×16mm chip with a reaction well, and they could work independently and be monitored simultaneously. A detect limit of 40pg/mL for ST2 antigen had been achieved, and the dynamic ranges for the three proteins detection were up to four orders of magnitude. The GMR sensing platform was also selective enough to be directly used in serum samples. In addition, a lab-based probe station has been designed to implement quick lab-on-a-chip experiments instead of wire bonding. It has a potential application in clinical biomarkers identification and screening, and can be extended to fit other biosensing schemes.

References

Feb 13, 2003·Circulation·Ellen O WeinbergRichard T Lee
Oct 4, 2005·Methods : a Companion to Methods in Enzymology·Jirí HomolaMarek Piliarik
Jul 3, 2009·Journal of Lipid Research·Geneviève DubucJean Davignon

❮ Previous
Next ❯

Citations

Apr 12, 2016·Frontiers in Microbiology·Venkatramana D KrishnaJian-Ping Wang
Jun 25, 2016·Sensors·María-Dolores Cubells-BeltránPaulo P Freitas
Jul 20, 2016·Analytical Chemistry·Jung-Rok LeeShan X Wang
Jun 20, 2018·Nanotechnology·Xueying ZhangWeisheng Zhao
Oct 27, 2015·PloS One·Anders Dahl HenriksenMikkel Fougt Hansen
May 23, 2019·Lab on a Chip·Jiri DietvorstAndreu Llobera
Jan 11, 2017·Nature Biotechnology·Emily Waltz
Sep 7, 2019·Nanotechnology·Kai WuJian-Ping Wang
Jan 1, 2020·Micromachines·Diqing SuJian-Ping Wang
Sep 16, 2020·Medicinal Research Reviews·Yatian FuBee Luan Khoo
Sep 11, 2020·Biosensors & Bioelectronics·Chih-Cheng HuangDrew A Hall
Oct 27, 2017·ACS Sensors·Kai WuJian-Ping Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.

Biomarkers for Cardiovascular Risk Assessment

Sensitive and accurate biomarkers used in cardiovascular risk prediction can potentially be used to manage the risk of cardiovascular disease. Discover the latest research on Biomarkers for Cardiovascular Risk Assessment here. Discover the latest research on Biomarkers for Cardiovascular Risk Assessment here.