Giardia duodenalis: protein substrates degradation by trophozoite proteases

Parasitology Research
Silvana Torossian Coradi, Semíramis Guimarães

Abstract

The present investigation was undertaken to identify and characterize trophozoite proteases of five axenic strains of Giardia duodenalis isolated in Brazil and the reference strain Portland 1 isolated in the United States. Trophozoite cell lysates of each strain were analysed for the pattern of proteins and for proteolytic activity. Samples were tested in SDS-polyacrylamide gel electrophoresis for the protein profiles, and the detection of proteases in cell lysates was performed using substrate gel electrophoresis [gelatin, collagen, bovine serum albumin (BSA) and haemoglobin] and azocasein assays. Indeed, synthetic inhibitors were included in the assays to characterize the protease classes. Differences on the hydrolysis patterns of protein substrates were observed in relation to the substrate composition as much as the Giardia trophozoite strain. The substrate-containing gels revealed hydrolysis bands with molecular masses ranging from >97 to 20-15 kDa, and most zones were common to the five strains. However, some pronounced differences could be detected in the BTU-11 pattern. Azocasein was also degraded; however, depending on the lysate assayed, the degree of substrate degradation was variable. It was observed that inhibitory...Continue Reading

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Citations

Oct 11, 2008·Parasitology Research·Haris Mirza, Kevin S W Tan
Jul 14, 2012·Journal of Parasitology Research·Carolina Piña-VázquezJesús Serrano-Luna
Sep 18, 2008·Parasitology Research·Thaís Batista de CarvalhoSemíramis Guimarães
Nov 16, 2010·Molecular and Biochemical Parasitology·B J DavidsF D Gillin
Feb 21, 2014·Revista Do Instituto De Medicina Tropical De São Paulo·Thaís Batista de CarvalhoSemíramis Guimarães
Oct 31, 2006·Memórias do Instituto Oswaldo Cruz·Guadalupe Beatriz Rodríguez-FuentesLilián Yépez-Mulia
Dec 25, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Erica Boarato DavidDiego Peres Alonso
Oct 6, 2020·Frontiers in Genetics·Dimitra PeirasmakiStaffan G Svärd
Jul 8, 2019·Trends in Parasitology·Thibault AllainAndre G Buret

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