Gibberellins regulate the abundance of RNAs with sequence similarity to proteinase inhibitors, dioxygenases and dehydrogenases

Planta
S E Jacobsen, N E Olszewski

Abstract

In an effort to understand the molecular mechanism of gibberellin (GA) action, we have cloned and performed an initial characterization of three cDNAs (GAD1, 2, and 3) which correspond to RNAs that become less abundant by 2 h after treatment of tomato (Lycopersicon esculentum Mill.) shoot tissue with gibberellic acid (GA3). Treatment with either auxin or ethephon also decreases the abundance of all three of the GAD RNAs. The tomato ethylene-insensitive mutant, Nr, and the GA-deficient mutant, gib1, were used to show that GA or auxin regulation of GAD RNA abundance is not dependent on ethylene sensitivity, and that ethylene or auxin regulation is not dependent on normal levels of gibberellin biosynthesis. Treatment with abscisic acid (ABA) antagonizes the GA-induced suppression of the GAD1 and GAD2 RNAs. GAD1 is similar to type-II wound-inducible plant proteinase inhibitors. Like the well-characterized proteinase inhibitor II (pin II) of tomato, the GAD1 and GAD2 RNAs are wound inducible. Induction of pin II and GAD1 RNA in gib1 was found to require less-severe wounding than was required using wild-type plants or plants doubly mutant for gib1 and sit (the sit mutation causes ABA deficiency). The predicted GAD2 protein sequence i...Continue Reading

Citations

Apr 25, 2001·The Plant Journal : for Cell and Molecular Biology·C W BachemR G Visser
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J C SedbrookP H Masson
May 28, 2013·Computational and Mathematical Methods in Medicine·Rekha KushwahaA Bruce Downie
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Irene OlimpieriAndrea Mazzucato
Nov 22, 2018·Journal of the Science of Food and Agriculture·Muhammad Umer FarooqJianqing Zhu
Sep 30, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sota KoedaYoshiyuki Tanaka

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