Gill-associated nidovirus of Penaeus monodon prawns transcribes 3'-coterminal subgenomic mRNAs that do not possess 5'-leader sequences

The Journal of General Virology
Jeff A CowleyPeter J Walker

Abstract

Sequence analysis of the approximately 20 kb 5'-terminal portion of the ssRNA genome of gill-associated virus (GAV) of Penaeus monodon prawns has previously established that it contains an ORF1a-1b replicase gene equivalent to those of the coronavirus and arterivirus members of the order Nidovirales. Sequence analysis of the remaining approximately 6.2 kb of the GAV genome downstream of ORF1a-1b to a 3'-poly(A) tail has identified two highly conserved intergenic sequences in which 29/32 nucleotides are conserved. Northern hybridization using probes to the four putative GAV ORFs and either total or poly(A)-selected RNA identified two 3'-coterminal subgenomic (sg) mRNAs of approximately 6 kb and approximately 5.5 kb. Primer extension and 5'-RACE analyses showed that the sgmRNAs initiate at the same 5'-AC positions in the central region of the two conserved intergenic sequences. Neither method provided any evidence that the GAV sgmRNAs are fused to genomic 5'-leader RNA sequences as is the case with vertebrate coronaviruses and arteriviruses. Intracellular double-stranded (ds)RNAs equivalent in size to the 26.2 kb genomic RNA and two sgRNAs were also identified by RNase/DNase digestion of total RNA from GAV-infected prawn tissue. ...Continue Reading

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Citations

Mar 12, 2010·Journal of Virological Methods·Priyanjalie K M WijegoonawardanePeter J Walker
Mar 1, 2006·Virus Research·Alexander E GorbalenyaEric J Snijder
Aug 13, 2005·Virus Research·Wai-Hong WuEric A Nelson
Dec 17, 2014·Virus Research·Kathleen C LehmannAlexander E Gorbalenya
Oct 30, 2016·Journal of Invertebrate Pathology·Eugene V Ryabov
Jun 29, 2018·Journal of Virology·Hazel StewartAndrew E Firth
Jun 16, 2011·MBio·Florian ZirkelSandra Junglen
Dec 28, 2002·Journal of Virology·John ZiebuhrAlexander E Gorbalenya
Jul 16, 2004·Journal of Virology·Alexander O PasternakEric J Snijder
Sep 22, 2006·Journal of Virology·Heike SchützeJohn Ziebuhr
Sep 1, 2010·Aquaculture Research·Yenelli Cedano-ThomasFrancisco Vargas-Albores
Jun 16, 2005·Journal of Virology·Saskia L SmitsRaoul J de Groot
May 13, 2006·The Journal of General Virology·Alexander O PasternakEric J Snijder
Jan 9, 2021·Biochemical and Biophysical Research Communications·Anastasia A Gulyaeva, Alexander E Gorbalenya
Apr 4, 2021·Viruses·Makoto Ujike, Fumihiro Taguchi
Sep 5, 2008·Virology·Priyanjalie K M WijegoonawardanePeter J Walker

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