Gingival crevicular fluid: As a diagnostic marker in HIV positive patients

Journal of International Society of Preventive & Community Dentistry
Prachi AtramSwati Gotmare

Abstract

To determine the utility of gingival crevicular fluid (GCF) for the detection of anti-HIV antibodies in human immunodeficiency virus (HIV)-infected individuals using enzyme linked immunosorbent assay (ELISA) with respect to their CD4 counts. The study was carried out between 37 seropositive (37GCF, 10 saliva) and 37 seronegative (GCF) individuals for a period of 7-8 months in GDC Nagpur. Thirty-seven GCF and 10 whole saliva samples were collected from the same patient. GCF samples were collected from gingival crevice with the help of Kimble disposable microcapillary. Saliva was collected by asking the patient to bend forward. The drooling saliva was collected in a sterile bottle and stored at Minus 20°C (-20°C). After the clinical observations the data were collected and tabulated for statistical analysis. When compared with serum, the sensitivity, specificity, and positive and negative predictive values of GCF were 100% respectively. All the above findings are suggestive of GCF being a better diagnostic medium than saliva.

References

Apr 1, 1993·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M UrquiaA Ceballos
Jan 1, 1993·The Journal of the American Dental Association·I D Mandel

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Citations

Dec 5, 2015·Frontiers in Cellular and Infection Microbiology·Simone FilardoRosa Sessa

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

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