Gingival Mesenchymal Stem Cells Outperform Haploidentical Dental Pulp-derived Mesenchymal Stem Cells in Proliferation Rate, Migration Ability, and Angiogenic Potential

Cell Transplantation
Ioannis AngelopoulosMaroun Khoury

Abstract

High donor variation makes comparison studies between different dental sources dubious. Dental tissues offer a rare opportunity for comparing the biological characteristics of haploidentical mesenchymal stem cells (MSCs) isolated from the same donor. The objective was to identify the optimal dental source of MSCs through a biological and functional comparison of haploidentical MSCs from gingival (GMSCs) and dental pulp stem cells (DPSCs) focusing mainly on their angiogenic potential. The comparison study included (1) surface markers expression, (2) mesodermal differentiation capacity (chondrogenic, adipogenic, and osteogenic), (3) proliferation, (4) migration potential, (5) ability to form colony units, and (6) angiogenic potential in vitro and in vivo. Comparative analysis showed no difference in the immunophenotypic profile nor for the trilineage differentiation potential. Proliferation of GMSCs was higher than DPSCs at day 6 (2.6-fold higher, P < 0.05). GMSCs showed superior migratory capacity compared to DPSCs at 4, 8, and 12 h (2.1-, 1.5-, and 1.2-fold higher, respectively, P < 0.05). Furthermore, GMSCs formed a higher number of colony units for both cell concentrations (1.7- and 1.4-fold higher for 150 and 250 starting ce...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Jun 24, 2006·Journal of Cellular Biochemistry·Reza IzadpanahBruce A Bunnell
Jun 4, 2008·Cancer Research·Reza IzadpanahBruce A Bunnell
Jun 19, 2008·Tissue Engineering. Part a·Morikuni TobitaHiroshi Mizuno
Aug 15, 2008·Cells, Tissues, Organs·Rachel J WaddingtonAlastair J Sloan
May 11, 2010·Journal of Periodontology·Tomas I MitranoAntonio Sanz
Sep 22, 2010·Journal of Cellular Physiology·Liang TangYan Jin
Jun 15, 2012·Biomaterials·Fa-Ming ChenQing Yu
Apr 22, 2014·Stem Cell Research·P HilkensA Bronckaers
Jun 1, 2014·The Journal of the American Dental Association·Martin B Steed
Oct 18, 2015·Stem Cell Research & Therapy·Francisca Alcayaga-MirandaMaroun Khoury

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Citations

Feb 28, 2020·Stem Cells and Cloning : Advances and Applications·Takeo W Tsutsui
Nov 9, 2019·Current Stem Cell Research & Therapy·Xudong Gao, Zhengguo Cao
Jan 24, 2019·International Journal of Molecular Sciences·Alina-Andreea ZimtaIoana Berindan-Neagoe
Apr 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qiaoqiao JinZhengwei Huang
Jun 25, 2020·International Journal of Molecular Sciences·Ji Won YangHyung-Sik Kim
Dec 2, 2020·European Journal of Dentistry·Alexander Patera NugrahaIgo Syaiful Ihsan
Mar 2, 2021·International Journal of Biological Macromolecules·Tong LeiHongwu Du
Apr 8, 2019·Archives of Oral Biology·Boon Chin HengChengfei Zhang

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Methods Mentioned

BETA
biopsies
flow cytometry
FACS
Assay
ELISA

Software Mentioned

Image J
ImageJ
FlowJo
WimScratch
WimTube

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