Abstract
High donor variation makes comparison studies between different dental sources dubious. Dental tissues offer a rare opportunity for comparing the biological characteristics of haploidentical mesenchymal stem cells (MSCs) isolated from the same donor. The objective was to identify the optimal dental source of MSCs through a biological and functional comparison of haploidentical MSCs from gingival (GMSCs) and dental pulp stem cells (DPSCs) focusing mainly on their angiogenic potential. The comparison study included (1) surface markers expression, (2) mesodermal differentiation capacity (chondrogenic, adipogenic, and osteogenic), (3) proliferation, (4) migration potential, (5) ability to form colony units, and (6) angiogenic potential in vitro and in vivo. Comparative analysis showed no difference in the immunophenotypic profile nor for the trilineage differentiation potential. Proliferation of GMSCs was higher than DPSCs at day 6 (2.6-fold higher, P < 0.05). GMSCs showed superior migratory capacity compared to DPSCs at 4, 8, and 12 h (2.1-, 1.5-, and 1.2-fold higher, respectively, P < 0.05). Furthermore, GMSCs formed a higher number of colony units for both cell concentrations (1.7- and 1.4-fold higher for 150 and 250 starting ce...Continue Reading
References
Apr 2, 1999·Science·M F PittengerD R Marshak
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Apr 1, 2003·Journal of Cell Science·Stan GronthosPaul J Simmons
Jun 24, 2006·Journal of Cellular Biochemistry·Reza IzadpanahBruce A Bunnell
Aug 23, 2006·Cytotherapy·M DominiciEm Horwitz
Jul 28, 2007·Stem Cells·Giselle ChamberlainJim Middleton
Dec 15, 2007·Stem Cells·Carolina GandiaPilar Sepúlveda
Feb 2, 2008·Stem Cells·Yi LiuSonglin Wang
Apr 2, 2008·Cell and Tissue Research·Wouter J F M JurgensFlorine J van Milligen
May 22, 2008·PloS One·Wolfgang WagnerAnthony D Ho
Jun 4, 2008·Cancer Research·Reza IzadpanahBruce A Bunnell
Jun 19, 2008·Tissue Engineering. Part a·Morikuni TobitaHiroshi Mizuno
Aug 15, 2008·Cells, Tissues, Organs·Rachel J WaddingtonAlastair J Sloan
Sep 22, 2009·Journal of Dental Research·G T-J HuangS Shi
Feb 9, 2010·Biochemical and Biophysical Research Communications·Geetanjali B TomarMohan R Wani
Apr 1, 2010·Oral Diseases·F FengS Shi
May 11, 2010·Journal of Periodontology·Tomas I MitranoAntonio Sanz
Sep 14, 2010·Biomaterials·Yi YangEdward E Putnins
Sep 22, 2010·Journal of Cellular Physiology·Liang TangYan Jin
Mar 3, 2011·Stem Cells and Development·Fang WangXuetao Pei
May 25, 2011·Biomaterials·Yuka TsumanumaYuichi Izumi
Jun 15, 2012·Biomaterials·Fa-Ming ChenQing Yu
Nov 21, 2012·Regenerative Medicine·Shaohua GeP Mark Bartold
Jun 19, 2013·Biomaterials·Hao YangFa-Ming Chen
Apr 22, 2014·Stem Cell Research·P HilkensA Bronckaers
Jun 1, 2014·The Journal of the American Dental Association·Martin B Steed
Apr 1, 2015·Cells, Tissues, Organs·Hyun Jin YangJong Won Rhie
Aug 15, 2015·Stem Cells Translational Medicine·Paz L GonzálezMaroun Khoury
Oct 18, 2015·Stem Cell Research & Therapy·Francisca Alcayaga-MirandaMaroun Khoury
Jan 1, 2012·Cell Medicine·Koji OtabeIchiro Sekiya
Citations
Oct 24, 2018·Cell Transplantation·JiaYang SunQingSan Zhu
Feb 28, 2020·Stem Cells and Cloning : Advances and Applications·Takeo W Tsutsui
Nov 9, 2019·Current Stem Cell Research & Therapy·Xudong Gao, Zhengguo Cao
Sep 24, 2020·Journal of Periodontal Research·Verónica M SolaAgata R Carpentieri
Jan 24, 2019·International Journal of Molecular Sciences·Alina-Andreea ZimtaIoana Berindan-Neagoe
Apr 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qiaoqiao JinZhengwei Huang
Jun 25, 2020·International Journal of Molecular Sciences·Ji Won YangHyung-Sik Kim
Dec 2, 2020·European Journal of Dentistry·Alexander Patera NugrahaIgo Syaiful Ihsan
Mar 2, 2021·International Journal of Biological Macromolecules·Tong LeiHongwu Du
Mar 26, 2021·Frontiers in Immunology·Christian Jorgensen, Maroun Khoury
Jun 5, 2021·ACS Biomaterials Science & Engineering·Sahar AnsariAlireza Moshaverinia
Jul 9, 2021·Stem Cells International·Gamilah Al-QadhiNiyaz Al-Sharabi
Apr 8, 2019·Archives of Oral Biology·Boon Chin HengChengfei Zhang
Nov 30, 2021·Advanced Science·Tian ZengWei Wu