Ginkgo biloba extract prevents against apoptosis induced by high glucose in human lens epithelial cells

Acta Pharmacologica Sinica
Zheng-mei WuJian-yun Wang

Abstract

To investigate the protective effects of Ginkgo biloba extract (GBE) on high glucose-induced apoptosis of human lens epithelial cells (HLEC) and the possible molecular mechanisms. The cultured HLEC were allotted into 6 groups: normal group, high glucose group, low-, moderate-, and high-dose GBE group, and the bendazac lysine group. Cell viability, cell apoptosis, the activities of cell antioxidases, aldose reductase, caspase-3, the levels of cell antioxidants, and the expressions of Bcl-2 and Bax were assessed by different methods. After being incubated with high glucose for 24 h, HLEC underwent apoptosis and exhibited significant oxidative stress. In the presence of GBE at different doses, the rate of HLEC apoptosis was lower and the oxidative stress state was significantly ameliorated. The increased ratio of Bax to Bcl-2 was significantly reduced and the activation of caspase-3 was suppressed by GBE in a dose-dependent manner. GBE prevents HLEC from high glucose-induced apoptosis through inhibiting oxidative stress, reducing the ratio of Bax to Bcl-2, and decreasing the activity of caspase-3. Therefore, GBE has a potential protective effect against diabetic cataract formation.

References

Aug 28, 1998·Science·D R Green, J C Reed
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Y Lee, S S Chung
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Aug 10, 2000·Trends in Cell Biology·S Desagher, J C Martinou
Oct 22, 2002·Experimental Eye Research·Geetha ThiagarajanDorairajan Balasubramanian
Nov 12, 2002·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Daniela VerzolaGiacomo Deferran
Dec 20, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kota V RamanaSatish K Srivastava
Jan 15, 2003·Experimental Physiology·Erich GulbinsJürgen Bock
Jul 23, 2003·Journal of the American Society of Nephrology : JASN·Stephen S M ChungSookja K Chung
May 4, 2004·Journal of Diabetes and Its Complications·Z KyselovaV Bauer
Sep 15, 2004·Molecular and Cellular Biochemistry·Suman BiswasDavid Phoenix
Feb 18, 2005·Diabetes, Obesity & Metabolism·K R Hegde, S D Varma
Jun 22, 2005·Experimental Eye Research·Seema SrivastavaSatish K Srivastava
Sep 20, 2005·The Journal of Nutritional Biochemistry·David A AllenSteven M Harwood
Apr 25, 2006·Experimental Eye Research·Agnieszka PladzykSatish K Srivastava
Apr 28, 2006·Investigative Ophthalmology & Visual Science·Namiki OishiYoshio Akagi
Aug 29, 2006·Investigative Ophthalmology & Visual Science·Michael L MulhernToshimichi Shinohara
Dec 13, 2006·Journal of Cell Science·Karen H Vousden
Feb 14, 2007·Japanese Journal of Ophthalmology·Masami KojimaKazuyuki Sasaki
Aug 21, 2007·Wiener medizinische Wochenschrift·Günter SiegelMartin Malmsten

❮ Previous
Next ❯

Citations

Feb 18, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Junghyun KimJin Sook Kim
Mar 18, 2011·World Journal of Gastroenterology : WJG·Glasiella Gonzalez Perez da SilvaNilza Cristina Buttow
Apr 17, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Xiao HanQi-Chang Yan
Mar 23, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Filipe MarquesRui Oliveira
Dec 6, 2017·International Journal of Molecular Medicine·Jike SongHongsheng Bi
Dec 10, 2021·Investigative Ophthalmology & Visual Science·Sheldon RowanAllen Taylor

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis