Ginkgo biloba extract suppresses endotoxin-mediated monocyte activation by inhibiting nitric oxide- and tristetraprolin-mediated toll-like receptor 4 expression

The Journal of Nutritional Biochemistry
Yuan-Wen LeeFeng-Yen Lin

Abstract

Monocytes expressing toll-like receptor 4 (TLR4) play a major role in regulating the innate immune response and are involved in systemic inflammation. Previous studies have shown that Ginkgo biloba extract (GBE) may act as a therapeutic agent for some cardiovascular and neurological disorders. The objective of this study was to determine whether GBE could modulate immunity in human cells. The monocytic cell line THP-1 was used. Enzyme-linked immunosorbent assay results showed that lipopolysaccharide (LPS) induces the expression of monocyte chemotactic protein-1 (MIP-1), tumor necrosis factor-α, stromal cell-derived factor-1, and MIP-1α, and this induction may be repressed by GBE treatment due to TLR4 blockade. The Griess reagent assay and western blot analysis showed that GBE-mediated inhibition of TLR4 expression was associated with the activation of mitogen-activated protein kinase and production of nitric oxide (NO). Actinomycin D chase experiments demonstrated that GBE decreased the TLR4 mRNA stability in cells. Confocal microscopy and real-time polymerase chain reaction showed that GBE induced the expression of intracellular tristetraprolin (TTP). Transfection with TTP siRNA reversed the effects of GBE in naïve or TLR4-ove...Continue Reading

References

Nov 7, 1992·Lancet·J Kleijnen, P Knipschild
Aug 15, 1995·European Journal of Biochemistry·G C Brown
Jul 17, 1995·Biochemical and Biophysical Research Communications·L J YanL Packer
Sep 8, 1995·The Journal of Biological Chemistry·M AsahiN Taniguchi
Jun 30, 1993·Biochemical and Biophysical Research Communications·R A Pufahl, M A Marletta
Jun 15, 1994·Biochemical and Biophysical Research Communications·L MarcocciL Packer
Sep 1, 1996·Circulation Research·R A KellyT W Smith
Oct 29, 1997·JAMA : the Journal of the American Medical Association·P L Le BarsA F Schatzberg
May 7, 1998·Metabolic Brain Disease·B K Sharma, K Kumar
Jan 19, 1999·Biochemistry and Molecular Biology International·S AkibaT Sato
Aug 12, 1999·Advances in Experimental Medicine and Biology·L R WatkinsS F Maier
Apr 12, 2000·Microbes and Infection·M Muzio, A Mantovani
Apr 18, 2000·Chest·S M Opal, V A DePalo
May 19, 2000·Cytokine & Growth Factor Reviews·T K MeansM J Fenton
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S ShohamS M Levitz
May 15, 2001·American Journal of Physiology. Cell Physiology·M F TsanJ E White
Jan 10, 2002·The Journal of Experimental Medicine·Christian TermeerJan Christoph Simon
Mar 26, 2002·The Journal of Biological Chemistry·Ramunas M VabulasHansjorg Schild
Jan 16, 2003·Immunology Letters·Shizuo Akira, Hiroaki Hemmi
Oct 3, 2003·Biochemical and Biophysical Research Communications·Packiasamy A R JulietLouis J Ignarro
Dec 9, 2003·The Journal of Biological Chemistry·Jong Sung ParkEdward Abraham
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bei LiuZihai Li
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geoffrey B JohnsonJeffrey L Platt
Jul 15, 2004·International Immunopharmacology·María Martha Villaseñor-GarcíaAna María Puebla-Pérez
Aug 24, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M G NeteaB J Kullberg
Aug 25, 2004·Experimental Eye Research·Iliyana IlievaShigeaki Ohno
Jul 30, 2005·International Journal of Cardiology·Mamoru SatohMotoyuki Nakamura
Nov 15, 2006·Current Pharmaceutical Design·Mihai G NeteaBart Jan Kullberg

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Citations

Nov 14, 2012·Phytotherapy Research : PTR·Dev S ChahalMohan R Dasu
Apr 26, 2016·The American Journal of Chinese Medicine·Rui JiaoKwok Fai So
Aug 21, 2019·Phytotherapy Research : PTR·Seyedeh Farzaneh OmidkhodaHossein Hosseinzadeh

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