GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks

Nature Cell Biology
Agnieszka GambusKarim Labib

Abstract

The components of the replisome that preserve genomic stability by controlling the progression of eukaryotic DNA replication forks are poorly understood. Here, we show that the GINS (go ichi ni san) complex allows the MCM (minichromosome maintenance) helicase to interact with key regulatory proteins in large replisome progression complexes (RPCs) that are assembled during initiation and disassembled at the end of S phase. RPC components include the essential initiation and elongation factor, Cdc45, the checkpoint mediator Mrc1, the Tof1-Csm3 complex that allows replication forks to pause at protein-DNA barriers, the histone chaperone FACT (facilitates chromatin transcription) and Ctf4, which helps to establish sister chromatid cohesion. RPCs also interact with Mcm10 and topoisomerase I. During initiation, GINS is essential for a specific subset of RPC proteins to interact with MCM. GINS is also important for the normal progression of DNA replication forks, and we show that it is required after initiation to maintain the association between MCM and Cdc45 within RPCs.

References

Dec 1, 1992·Molecular and Cellular Biology·N KouprinaF Spencer
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Miles, T Formosa
Jul 20, 1989·Journal of Molecular Biology·R A Kim, J C Wang
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L YangL F Liu
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R WobbeJ Hurwitz
Aug 15, 1996·Virology·D T SimmonsB Stillman
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·S DonovanJ F Diffley
Apr 5, 2001·Molecular and Cellular Biology·J S HannaF A Spencer
Jun 21, 2001·Molecular and Cellular Biology·K YoshidaA Dutta
Nov 21, 2001·Nature Cell Biology·A A AlcasabasS J Elledge
Nov 21, 2001·Nature Cell Biology·K Tanaka, P Russell
May 6, 2003·Genes & Development·Yumiko KubotaHaruhiko Takisawa
Aug 23, 2003·Science·Rimma BelotserkovskayaDanny Reinberg
Jan 27, 2004·Molecular Biology of the Cell·Melanie L MayerPhilip Hieter
Feb 10, 2004·The Journal of Biological Chemistry·Karen FienJerard Hurwitz
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Susan L Forsburg
Aug 25, 2004·The Journal of Biological Chemistry·David ShechterJean Gautier
Aug 27, 2004·The EMBO Journal·Marcin Pacek, Johannes C Walter
Sep 16, 2004·Molecular and Cellular Biology·Eishi NoguchiPaul Russell
Oct 21, 2004·Molecular Cell·Robin M Ricke, Anja-Katrin Bielinsky
Aug 18, 2005·Nucleic Acids Research·Kira S MakarovaEugene V Koonin

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Citations

Sep 21, 2012·Chromosoma·Jeffrey P ChmielewskiTim W Christensen
Feb 16, 2013·Chromosoma·Silvia Onesti, Stuart A MacNeill
Dec 18, 2012·Archives of Virology·Geetika SuyalNirupam R Choudhury
Jun 10, 2011·Extremophiles : Life Under Extreme Conditions·Hiromi OginoYoshizumi Ishino
Dec 30, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sandie TuduriPhilippe Pasero
Feb 24, 2010·Nature Reviews. Molecular Cell Biology·Dana Branzei, Marco Foiani
Feb 24, 2012·Nature Reviews. Molecular Cell Biology·Constance Alabert, Anja Groth
Mar 8, 2011·Nature Structural & Molecular Biology·Alessandro CostaJames M Berger
Dec 6, 2011·Nature Structural & Molecular Biology·Yoshitami HashimotoVincenzo Costanzo
Jul 11, 2006·Nature Structural & Molecular Biology·Megan G ClareyMichael Botchan
Apr 10, 2007·Nature Structural & Molecular Biology·Katsuhiko KamadaFumio Hanaoka
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Naoki NitaniTakuro Nakagawa
Jun 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maria L NaylorStephen J Elledge
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jun-Sub ImJoon-Kyu Lee
Nov 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cecile EvrinChristian Speck
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nadia GurvichStephen D Nimer
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Young-Hoon KangJerard Hurwitz
Aug 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ivar IlvesMichael R Botchan
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Won-Ho ChoJoon-Kyu Lee
May 22, 2009·The Journal of Biological Chemistry·Sheng-Hong Chen, Huilin Zhou
Jul 18, 2009·The Journal of Biological Chemistry·Eric M WarrenBrandt F Eichman
Aug 21, 2009·The Journal of Biological Chemistry·Irina Bruck, Daniel Kaplan
Oct 13, 2009·The Journal of Biological Chemistry·Masashige BandoKatsuhiko Shirahige
Mar 2, 2010·The Journal of Biological Chemistry·Daniel A Gold, William G Dunphy
Feb 9, 2010·The Journal of Biological Chemistry·Satoru MimuraTakumi Kamura
Jan 22, 2010·The Journal of Biological Chemistry·Vladimir P BermudezJerard Hurwitz
May 22, 2010·The Journal of Biological Chemistry·Patrick D RobertsonBrandt F Eichman
Oct 7, 2010·The Journal of Biological Chemistry·Taku TanakaHisao Masai
Feb 2, 2011·The Journal of Biological Chemistry·Agnieszka GambusJ Julian Blow
Mar 3, 2011·The Journal of Biological Chemistry·Irina Bruck, Daniel L Kaplan
Jun 28, 2011·The Journal of Biological Chemistry·Vladimir P BermudezJerard Hurwitz
May 18, 2012·The Journal of Biological Chemistry·Mairi L KilkennyLuca Pellegrini
Sep 20, 2008·The Journal of Biological Chemistry·Diane M KanterDaniel L Kaplan
May 15, 2009·The Journal of Biological Chemistry·Naoko Yoshizawa-Sugata, Hisao Masai
Dec 25, 2008·Molecular & Cellular Proteomics : MCP·Jean-Philippe LambertDaniel Figeys
Aug 19, 2007·Molecular Biology of the Cell·Sharbani Chattopadhyay, Anja-Katrin Bielinsky
Jul 27, 2007·Molecular Biology of the Cell·Ben HodgsonKarim Labib

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