Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil

Journal of Ginseng Research
Chong-Zhi WangChun-Su Yuan

Abstract

Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regulation mechanisms. Ginseng berry polysaccharide extract (GBPE) and purified ginseng berry polysaccharide portion (GBPP) were used to evaluate their activities on human HCT-116 and HT-29 CRC cell proliferation. Interleukin-8 secretion analysis was performed on HT-29 cells. Naive CD4 cell isolation and T-helper cell differentiation were performed and determined using flow cytometry for Th1 and Treg in addition to cell cycle and apoptotic investigation. GBPE and GBPP significantly inhibited interleukin-8 secretion and cancer cell proliferation, inhibited CD4+IFN-γ+ cell (Th1) differentiation, and decreased CD4+FoxP3+ cell (Treg) differentiation. Compared to the GBPE, GBPP showed more potent antiinflammatory activities on the malignant cells. This is consistent with the observation that GBPP can also inhibit Th1-cell differentiation better, suggesting that it has an important role in antiinflammation, whereas Treg cells hinder th...Continue Reading

Citations

Dec 18, 2020·Food & Function·Mingkun GuoMingxing Wang
Apr 27, 2021·Molecular and Cellular Biochemistry·Do Luong HuynhCuong Thach Nguyen
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hongyu QiXiangyan Li

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PMA
flow cytometry

Software Mentioned

FlowJo

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