Ginseng Extract Modified by Pectin Lyase Inhibits Retinal Vascular Injury and Blood-Retinal Barrier Breakage in a Rat Model of Diabetes

Journal of Medicinal Food
Eunsoo JungJunghyun Kim

Abstract

GS-E3D is an enzymatically modified ginseng extract by pectin lyase. In this study, we evaluated the preventive effects of GS-E3D on blood-retinal barrier (BRB) leakage in a rat model of diabetes. To produce diabetes, rats were injected with streptozotocin. GS-E3D was orally gavaged at 25, 50, and 100 mg/kg body weight for 6 weeks. We then compared the effect of GS-E3D with that of an unmodified ginseng extract (UGE) on retinal vascular leakage. The administration of GS-E3D significantly blocked diabetes-induced BRB breakdown. Immunofluorescence staining showed that GS-E3D reduced the loss of occludin in diabetic rats. In TUNEL staining, the number of apoptotic retinal microvascular cells was dose dependently decreased by GS-E3D treatment. GS-E3D decreased the accumulations of advanced glycation end products in the retinal vessels. In addition, the inhibition potential of GS-E3D on BRB breakage was stronger compared with UGE. These results indicate that GS-E3D could be a beneficial treatment option for preventing diabetes-induced retinal vascular injury.

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H P HammesM Brownlee
Oct 1, 1989·Diabetes·R L Engerman
Jan 1, 1995·Annual Review of Medicine·M Brownlee
Jan 27, 1998·Journal of Diabetes and Its Complications·R S ClementsM P Cohen
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·B H WolffenbuttelB I Lévy
Aug 26, 1998·The Journal of Clinical Investigation·A J BarberT W Gardner
Sep 2, 1999·Biochemical and Biophysical Research Communications·S RahbarI Lalezari
Oct 6, 2000·American Journal of Respiratory Cell and Molecular Biology·M KasperE D Schleicher
Oct 18, 2000·Molecular Cell Biology Research Communications : MCBRC·A W StittD B Archer
Jan 19, 2002·Biochemical and Biophysical Research Communications·Sho-ichi YamagishiZenji Makita
Jan 29, 2002·American Journal of Ophthalmology·Donald S FongPaul P Lee
Jan 31, 2003·Investigative Ophthalmology & Visual Science·Yuichi KajiSeiko Horiuchi
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Tara C B MooreAnthony P Adamis
Mar 24, 2004·Diabetes/metabolism Research and Reviews·José Pedro De La CruzFelipe Sánchez de la Cuesta
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Nicole S Harhaj, David A Antonetti
Jan 19, 2005·Journal of Pharmacological Sciences·Ji Hyun KimInsop Shim
Apr 13, 2005·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·T-P LiuJ-T Cheng
May 26, 2005·Pharmacological Reviews·Brian T Hawkins, Thomas P Davis
Jul 23, 2005·Annals of the New York Academy of Sciences·Toshihiko Osawa, Yoji Kato
May 23, 2006·Current Drug Discovery Technologies·Sho-ichi YamagishiTakanori Matsui
Nov 1, 2006·International Journal of Clinical Practice·A GirachM Porta
Mar 21, 2009·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Yan GuoYun-lin Cheng
Sep 30, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Houfu LiuChuan Li
Apr 9, 2010·Applied Microbiology and Biotechnology·Chang-Su ParkDeok-Kun Oh
Oct 30, 2010·Circulation Research·Ferdinando Giacco, Michael Brownlee
Mar 10, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Souren PaulSun Chul Kang
May 26, 2012·Bioorganic & Medicinal Chemistry Letters·Dawei LuoXun Xu
Aug 29, 2012·Journal of Ethnopharmacology·Young Joo HongKyung-Mi Lee
Oct 30, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pitna KimChan Young Shin
May 30, 2013·Journal of Ginseng Research·Hai Yan QuanSung Hyun Chung
Oct 12, 2013·Molecular Nutrition & Food Research·María Ángeles MartínLuis Goya

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Citations

Jan 16, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eunsoo JungJunghyun Kim

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Methods Mentioned

BETA
fluorescence microscopy

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GraphPad
Prism
Image J

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