Ginsenoside Rb1 exerts anti-inflammatory effects in vitro and in vivo by modulating toll-like receptor 4 dimerization and NF-kB/MAPKs signaling pathways

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Hongwei GaoShilin Yang

Abstract

Ginsenoside Rb1, the main active constituent of Panax ginseng, displays significant anti-inflammatory activity, although the mechanism has not been clearly unraveled. In this study, Rb1's mechanism of anti-inflammatory effects were investigated. The flow cytometry and enzyme-linked immunosorbent assay (ELISA) were empolyed to detect pro-inflammatory cytokines release. The related protein and gene expression was investigated by western blotting and qRT-PCR. The dimerization of TLR4 was measured by co-immunoprecipitation and molecular docking assays. Cellular thermal shift assay was used for the determination of the binding of Rb1 and TLR4. For animal moldels, LPS- or cantharidin-induced acute kidney injury, LPS-induced septic death, and dimethyl benzene-induced ear edema were employed to investigate Rb1's anti-inflammatory activity in vivo. Rb1 significantly decreased inflammatory cytokines release in LPS-stimulated RAW264.7 cells and BMDMs, as well as COX-2 and iNOS amounts. Rb1 reduced LPS-associated calcium influx, ROS production, and NO generation. The NF-κB and MAPK axes participated in Rb1's anti-inflammatory effects. Molecular docking simulation indicated Rb1 bound to TLR4 to prevent TLR4 dimerization, as confirmed by co-...Continue Reading

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Citations

Nov 20, 2020·ChemMedChem·Lea WinandMarkus Nett
Nov 6, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tianmu HeJianyong Zhang
Feb 23, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Cai-Rong ZhangGuang-Xia Ni
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei ChenDavid G Popovich
Apr 16, 2021·Frontiers in Pharmacology·Mingming ZhaoYu Zhang
Jul 13, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Haijuan ZhiWanying Wu

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