Ginsenoside Re Attenuates High Glucose-Induced RF/6A Injury via Regulating PI3K/AKT Inhibited HIF-1α/VEGF Signaling Pathway

Frontiers in Pharmacology
Weijie XieXiaobo Sun

Abstract

Hyperglycaemia-induced retinal microvascular endothelial cell apoptosis is a critical and principle event in diabetic retinopathy (DR), which involves a series of complex processes such as mitochondrial dysfunction and oxidative stress. Ginsenoside Re (Re), a key ingredients of ginseng, is considered to have various pharmacologic functions, such as antioxidative, inhibition of inflammation and anti-apoptotic properties. However, the effects of Re in DR and the related mechanisms of endothelial cell injury induced by high glucose (HG) exposure remain unclear. The present study was designed to investigate and evaluate the ability of Re to ameliorate HG-induced retinal endothelial RF/6A cell injury and the potential mechanisms involved in the hypoxia-inducible factor-1-alpha (HIF-1α)/vascular endothelial growth factor (VEGF) signaling regulated by phosphoinositide 3-kinase (PI3K)/AKT pathway. Our results showed that preincubation with Re exerted cytoprotective effects by reversing the HG-induced decrease in RF/6A cell viability, downregulation of apoptosis rate and inhibition of oxidative-related enzymes, thereby reducing the excess intracellular reactive oxygen species (ROS) and HG-triggered RF/6A cell injury. In addition, Wester...Continue Reading

References

Oct 1, 1972·The American Journal of Clinical Nutrition·J LindenbaumC D Gerson
Jun 14, 2005·Biochimica Et Biophysica Acta·Jing-Tian XieChun-Su Yuan
Jul 21, 2007·Experimental Diabetes Research·Renu A Kowluru, Pooi-See Chan
Oct 24, 2008·American Journal of Physiology. Cell Physiology·Qiong Huang, Nader Sheibani
Mar 11, 2010·American Journal of Physiology. Endocrinology and Metabolism·Yukiko MinamiyamaShigeru Okada
Oct 11, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yi JingBing-Hua Jiang
Apr 25, 2012·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Gabriele E Lang
Apr 23, 2013·Life Sciences·Paulo Zoé Costa, Raquel Soares
Jun 8, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Taiyoun RhimMinhyung Lee
May 31, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Dong Hyun JoJeong Hun Kim
Aug 28, 2014·Diabetes, Obesity & Metabolism·A DasS Rangasamy
Nov 15, 2015·The Journal of Pharmacology and Experimental Therapeutics·Eryun ZhangZhengtao Wang
May 28, 2016·Circulation Research·Joshua A Beckman, Mark A Creager
Jan 4, 2017·Diabetes & Metabolic Syndrome·Mohsen MazidiSophie Siest
Mar 3, 2017·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Jun-Hui DuLe Ma
Aug 25, 2017·Current Diabetes Reports·Ana Maria OlivaresNeena Haider
Dec 9, 2017·Nature Reviews. Endocrinology·Yan ZhengFrank B Hu
Mar 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guang-Jiang ShiJian-Qiang Yu
Apr 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weijie XieXiaobo Sun
May 29, 2018·International Journal of Endocrinology·Sonia Sifuentes-FrancoAlejandra Guillermina Miranda-Díaz
Jul 24, 2018·Pakistan Journal of Medical Sciences·Farhan Khashim Alswailmi
Sep 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiangbao MengXiaobo Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
MDA
fluorescence microscopy
nuclear translocation

Software Mentioned

Prism
Image J

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis