Ginsenoside Rg1 activated CaMKIIalpha mediated extracellular signal-regulated kinase/mitogen activated protein kinase signaling pathway

Acta Pharmacologica Sinica
Jin-Feng HuNai-Hong Chen

Abstract

We carried out this study to investigate the effect of ginsenoside Rg1 on the extracellular signal-regulated kinase/mitogen activated protein kinase (ERK/ MAPK) pathway for understanding its effect on synaptic platicity. Western blotting and immunostaining were used to examine the phosphorylation of ERK1/2, CaMKIIalpha and cAMP response element binding protein (CREB) in PC12 cells and synaptosomes. The confocal microscopy and fluorescent indicator Fluo-3 was applied to observe the intracellular calcium ion flux. The phosphorylation of ERK1/2 in PC12 cells and synaptosomes incubated with Rg1 was increased and reached maximum at 4 min. Rg1 also promoted the transient enhancement of upstream calcium ion and activated CaMKIIalpha, which reached maximum at 2 min. CREB, the downstream protein, was phosphorylated within 8 min in PC12 cells after being incubated with Rg1. Moreover, KN93 partially inhibited the activation of ERK1/2, and PD98059 also partially blocked the phosphorylation of CREB. Rg1 activated ERK/MAPK pathway by CaMKIIalpha, and the activation of CREB was not only dependent on ERK induced by Rg1, which may provide an explanation for the effect of Rg1 on long-term potentiation.

References

Oct 4, 1996·The Journal of Biological Chemistry·J D English, J D Sweatt
Aug 1, 1997·The Journal of Biological Chemistry·J D English, J D Sweatt
Nov 26, 1999·Biochemical Pharmacology·A S AtteleC S Yuan
Jun 29, 2000·Annual Review of Biochemistry·A J Shaywitz, M E Greenberg
Oct 19, 2002·Progress in Neurobiology·John Bradley, Steven Finkbeiner
Mar 26, 2004·Life Sciences·Koichi Obata, Koichi Noguchi
Jun 15, 2004·Current Opinion in Neurobiology·J David Sweatt
Jan 25, 2005·Acta Pharmacologica Sinica·Yong ChengJun-Tian Zhang
Nov 7, 2006·Current Opinion in Pharmacology·Jean-Antoine GiraultDenis Hervé
Dec 16, 2006·Progress in Brain Research·Sara C ShalinJ David Sweatt
Nov 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sivan Ida Cohen-MatsliahEdi Barkai

❮ Previous
Next ❯

Citations

Jan 19, 2010·Chinese Journal of Integrative Medicine·Shi-feng Chu, Jun-tian Zhang
Jun 18, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zheng MouJun-Tian Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.