Ginsenoside Rg1 protects human fibroblasts against psoralen- and UVA-induced premature senescence through a telomeric mechanism

Archives of Dermatological Research
Bing-Rong ZhouDan Luo

Abstract

This study was aimed to investigate the protective effects of ginsenoside Rg1 on 8-methoxypsoralen(8-MOP)/Ultraviolet A (UVA)-induced premature senescence in human fibroblasts, and the underlying mechanism. We established a stress-induced premature senescence model by 8-MOP/UVA irradiation. The aging condition was determined by histochemical staining of senescence-associated β-galactosidase (SA-β-gal). Relative telomere length was calculated by the ratio of the amount of telomere DNA versus single copy DNA by real-time polymerase chain reaction, and protein levels of p-P53, p21(WAF-1) and p16(INK-4a) were estimated by Western blotting. Compared with the 8-MOP/UVA treatment group, we found that the irradiated fibroblasts pretreated with ginsenoside Rg1 demonstrated a decrease in the expression of SA-β-gal, a downregulation in the level of senescence-associated proteins, and a deceleration in telomere shortening. Taken together, these results suggest that ginsenoside Rg1 significantly antagonizes premature senescence induced by 8-MOP/UVA in fibroblasts.

References

Dec 1, 1996·The British Journal of Dermatology·B A Gilchrest
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Aug 24, 1999·BioTechniques·J L BoulayC Rochlitz
Jun 15, 2000·Experimental Cell Research·J SeverinoV J Cristofalo
Dec 10, 2002·Experimental Gerontology·Wenjian MaKarin Scharffetter-Kochanek
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·M G Kosmadaki, B A Gilchrest
May 8, 2004·Antioxidants & Redox Signaling·Chikako NishigoriShinya Toyokuni
Jul 13, 2004·Annals of the New York Academy of Sciences·Shosuke Kawanishi, Shinji Oikawa
Jan 25, 2005·Acta Pharmacologica Sinica·Yong ChengJun-Tian Zhang
Apr 1, 2008·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Xiaochun ChenYuangui Zhu

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Citations

Aug 29, 2013·International Journal of Biological Sciences·Bing-rong ZhouDan Luo
Apr 26, 2016·Molecular Medicine Reports·Yajun GuXiaochun Sun
Nov 16, 2020·Journal of Ethnopharmacology·Mengjiao HeChunchao Han

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