Ginsenoside Rg3 Suppresses Proliferation and Induces Apoptosis in Human Osteosarcoma

BioMed Research International
Yi LiZiming Dong

Abstract

Osteosarcoma is the most common primary malignancy of bone in children and the elderly. Recently, more and more researches have demonstrated that Ginsenoside Rg3 (Rg3) is involved in chemotherapy resistance in many cancer, making it a promising Chinese herbal monomer for oncotherapy. In this study, we investigated the efficacy of Rg3 in human osteosarcoma cell lines (MG-63, U-2OS, and SaOS-2). Cell proliferation was measured by CCK8 assay. The migration of cells was examined using the scratch assay method. Quantification of apoptosis was assessed further by flow cytometry. In addition, the expression of apoptosis-related genes (caspase9, caspase3, Bcl2, and Bax) were investigated using RT-PCR. We further investigated the protein level expression of Bcl 2, cleaved-caspase3, and PI3K/AKT/mTOR signaling pathway factors by Western blot assay. Our results revealed that Rg3 inhibited the proliferation and migration of human osteosarcoma cells and induced apoptosis in a concentration- and time-dependent manner. Western blot results showed that Rg3 reduced the protein expression of Bcl2 and PI3K/AKT/mTORbut increased the levels of cleaved-caspase3. Therefore, we hypothesized Rg3 inhibits the proliferation of osteosarcoma cell line and ...Continue Reading

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Citations

Oct 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuai BianDean Guo
Aug 1, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaohan MaoGuowei Zuo
Mar 5, 2021·Molecular Biology Reports·Zongyu LiuKai Zhang

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Methods Mentioned

BETA
electrophoresis
flow cytometry
PCR
flow
surgical resection

Software Mentioned

CFlow
Microplate Manager
SPSS

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