Gintonin stimulates autophagic flux in primary cortical astrocytes

Journal of Ginseng Research
Md Ataur RahmanHyewhon Rhim

Abstract

Gintonin (GT), a novel ginseng-derived exogenous ligand of lysophosphatidic acid (LPA) receptors, has been shown to induce cell proliferation and migration in the hippocampus, regulate calcium-dependent ion channels in the astrocytes, and reduce β-amyloid plaque in the brain. However, whether GT influences autophagy in cortical astrocytes is not yet investigated. We examined the effect of GT on autophagy in primary cortical astrocytes using immunoblot and immunocytochemistry assays. Suppression of specific proteins was performed via siRNA. LC3 puncta was determined using confocal microscopy. GT strongly upregulated autophagy marker LC3 by a concentration- as well as time-dependent manner via G protein-coupled LPA receptors. GT-induced autophagy was further confirmed by the formation of LC3 puncta. Interestingly, on pretreatment with an mammalian target of rapamycin (mTOR) inhibitor, rapamycin, GT further enhanced LC3-II and LC3 puncta expression. However, GT-induced autophagy was significantly attenuated by inhibition of autophagy by 3-methyladenine and knockdown Beclin-1, Atg5, and Atg7 gene expression. Importantly, when pretreated with a lysosomotropic agent, E-64d/peps A or bafilomycin A1, GT significantly increased the leve...Continue Reading

Citations

May 12, 2020·Frontiers in Cell and Developmental Biology·Md Ataur RahmanSsang-Goo Cho
Oct 3, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Md JakariaDong-Kug Choi
Aug 21, 2020·Integrative Medicine Research·Sun-Hye ChoiSeung-Yeol Nah

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Methods Mentioned

BETA
transgenic
confocal microscopy
transfection
protein assay
electrophoresis
gene knockdown

Software Mentioned

Leica Application Suite X ( LAS
Prism
AlphaEase
Image J
GraphPad

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