Gi/o protein-coupled receptors inhibit neurons but activate astrocytes and stimulate gliotransmission

Glia
Caitlin A DurkeeAlfonso Araque

Abstract

G protein-coupled receptors (GPCRs) play key roles in intercellular signaling in the brain. Their effects on cellular function have been largely studied in neurons, but their functional consequences on astrocytes are less known. Using both endogenous and chemogenetic approaches with DREADDs, we have investigated the effects of Gq and Gi/o GPCR activation on astroglial Ca2+ -based activity, gliotransmitter release, and the functional consequences on neuronal electrical activity. We found that while Gq GPCR activation led to cellular activation in both neurons and astrocytes, Gi/o GPCR activation led to cellular inhibition in neurons and cellular activation in astrocytes. Astroglial activation by either Gq or Gi/o protein-mediated signaling stimulated gliotransmitter release, which increased neuronal excitability. Additionally, activation of Gq and Gi/o DREADDs in vivo increased astrocyte Ca2+ activity and modified neuronal network electrical activity. Present results reveal additional complexity of the signaling consequences of excitatory and inhibitory neurotransmitters in astroglia-neuron network operation and brain function.

References

Feb 1, 1989·Trends in Biochemical Sciences·H Schulman, L L Lou
Sep 1, 1989·British Journal of Pharmacology·R A North
Aug 1, 1995·Current Opinion in Neurobiology·G Buzsáki, J J Chrobak
Aug 1, 1996·Trends in Neurosciences·A Verkhratsky, H Kettenmann
Jan 1, 1997·Annual Review of Biochemistry·W D SingerP C Sternweis
Sep 30, 1998·The European Journal of Neuroscience·A AraqueP G Haydon
Apr 10, 1999·Nature Neuroscience·J KangM Nedergaard
May 14, 1999·Trends in Neurosciences·A AraqueP G Haydon
Aug 26, 2000·Progress in Biophysics and Molecular Biology·B S Brown, S P Yu
Feb 22, 2001·Annual Review of Physiology·A AraqueP G Haydon
Aug 3, 2001·Nature Reviews. Neuroscience·E Salinas, T J Sejnowski
Sep 5, 2001·The Journal of Physiology·D EngelA Draguhn
Aug 6, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·María Cecilia AnguloEtienne Audinat
Mar 5, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gertrudis Perea, Alfonso Araque
Jul 19, 2005·Nature Reviews. Neuroscience·Andrea Volterra, Jacopo Meldolesi
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jane E LaucknerKen Mackie
Feb 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sonia Gasparini, Jeffrey C Magee
May 19, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexandre SerranoRichard Robitaille
Jul 4, 2006·Physiological Reviews·Philip G Haydon, Giorgio Carmignoto
Dec 21, 2006·Nature Reviews. Neuroscience·Marlene BartosPeter Jonas
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Blaine N ArmbrusterBryan L Roth
Mar 28, 2008·Neuron·Marta Navarrete, Alfonso Araque
May 9, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeremy PetraviczKen D McCarthy
May 16, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cary L ScheidererLori L McMahon
Jun 27, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eiji ShigetomiBaljit S Khakh
Jul 21, 2009·Trends in Neurosciences·Gertrudis PereaAlfonso Araque
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Tommaso FellinPhilip G Haydon
Feb 13, 2010·Annual Review of Physiology·Michael M Halassa, Philip G Haydon
Dec 17, 2010·Journal of Neurophysiology·Sameera Dasari, Allan T Gulledge
Jun 4, 2011·Current Opinion in Neurobiology·Dimitri M Kullmann
Sep 13, 2011·Nature Neuroscience·Maria Amalia Di CastroAndrea Volterra

❮ Previous
Next ❯

Citations

Dec 28, 2019·Glia·Alastair J MacDonaldKate L J Ellacott
May 20, 2020·Journal of Neuroscience Research·Kevin UngBenjamin Deneen
Feb 11, 2020·Frontiers in Cardiovascular Medicine·Anais AudebrandCanan G Nebigil
Sep 10, 2020·Life Science Alliance·Najwa Ouali AlamiFrancesco Roselli
Sep 3, 2020·Science Advances·Lihong ShengRoberto Bonasio
Jul 16, 2020·Experimental & Molecular Medicine·Keebum Park, Sung Joong Lee
Sep 3, 2020·Nature Reviews. Neuroscience·Alexey SemyanovAmit Agarwal
Feb 12, 2020·Nature Reviews. Neuroscience·Xinzhu YuBaljit S Khakh
Dec 3, 2020·International Journal of Molecular Sciences·Álvaro Fernández-Blanco, Mara Dierssen
Dec 20, 2020·Immunobiology·Valentina G SafronovaVictor I Tsetlin
Apr 1, 2020·Neuroscience·Paulo Kofuji, Alfonso Araque
Feb 11, 2021·PLoS Computational Biology·Arash MoshkforoushJorge Riera
Dec 17, 2020·Frontiers in Cellular Neuroscience·Laura C CaudalFrank Kirchhoff
Sep 17, 2020·Science Advances·Lihong ShengRoberto Bonasio
Apr 11, 2021·Neurochemical Research·José Antonio Noriega-Prieto, Alfonso Araque
Apr 11, 2021·Molecular Psychiatry·Junshi WangYan Dong
Apr 25, 2021·Progress in Neurobiology·Matthew J Broadhead, Gareth B Miles
Jun 4, 2021·Neuroscience Bulletin·Yi-Hua Chen, Tian-Ming Gao
Jun 15, 2021·Frontiers in Psychiatry·James TangGiannina Descalzi
Jun 25, 2021·Frontiers in Cellular Neuroscience·Annamaria LiaGabriele Losi
Jun 25, 2021·Frontiers in Cellular Neuroscience·Eunice Y LimMartin Paukert
Jan 7, 2021·Annual Review of Neuroscience·Paulo Kofuji, Alfonso Araque
Jul 14, 2021·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Michelle Corkrum, Alfonso Araque
Aug 3, 2021·Trends in Neurosciences·Caitlin DurkeeAlfonso Araque
Nov 6, 2021·Scientific Reports·P ÓrdenesMaría A García-Robles

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

The International Journal of Biochemistry & Cell Biology
Arun K Shukla
Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
F ToumiS J Keely
© 2022 Meta ULC. All rights reserved