Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.

PloS One
Jakub Orzechowski WestholmHans Ronne

Abstract

Aging in organisms as diverse as yeast, nematodes, and mammals is delayed by caloric restriction, an effect mediated by the nutrient sensing TOR, RAS/cAMP, and AKT/Sch9 pathways. The transcription factor Gis1 functions downstream of these pathways in extending the lifespan of nutrient restricted yeast cells, but the mechanisms involved are still poorly understood. We have used gene expression microarrays to study the targets of Gis1 and the related protein Rph1 in different growth phases. Our results show that Gis1 and Rph1 act both as repressors and activators, on overlapping sets of genes as well as on distinct targets. Interestingly, both the activities and the target specificities of Gis1 and Rph1 depend on the growth phase. Thus, both proteins are associated with repression during exponential growth, targeting genes with STRE or PDS motifs in their promoters. After the diauxic shift, both become involved in activation, with Gis1 acting primarily on genes with PDS motifs, and Rph1 on genes with STRE motifs. Significantly, Gis1 and Rph1 control a number of genes involved in acetate and glycerol formation, metabolites that have been implicated in aging. Furthermore, several genes involved in acetyl-CoA metabolism are downregu...Continue Reading

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Citations

Sep 26, 2013·Oxidative Medicine and Cellular Longevity·Ivan OrlandiMarina Vai
Dec 19, 2013·PLoS Computational Biology·Sriram Chandrasekaran, Nathan D Price
May 29, 2013·Genome Biology·Mingguang LiJeffrey S Smith
Jun 17, 2015·Mechanisms of Ageing and Development·Kwanjeera WanichthanarakDina Petranovic
Feb 6, 2019·Frontiers in Physiology·Niek WelkenhuysenMarija Cvijovic

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Datasets Mentioned

BETA
E-TABM-496

Methods Mentioned

BETA
histone acetylation
histone
dissection
PCR

Software Mentioned

ArrayExpress
Bioconductor
MEV
Affy
SGD
BioProspector
RSAT

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