GISH, AFLP and PCR-RFLP analysis of an intergeneric somatic hybrid combining Goutou sour orange and Poncirus trifoliata

Plant Cell Reports
C H FuX X Deng

Abstract

Intergeneric somatic hybrids combining Goutou sour orange (Citrus aurantium L.) with trifoliate orange Poncirus trifoliata (L.) Raf] were produced by electrofusion and their genetic inheritance analyzed by amplified fragment length polymorphism (AFLP), genomic in situ hybridization (GISH), and PCR-restriction fragment length polymorphism (PCR-RFLP). Sixteen mini-calluses were obtained after 20 days of culture; they all developed into embryoids on EME500 medium. Following several subcultures on shoot induction medium for a total culture period of 6 months, shoots regenerated. The plants grew vigorously with a well-developed root system and exhibited the trifoliate leaf character of P. trifoliata. Ploidy analysis verified that all of the regenerates were tetraploids (2 n=4 x=36) as expected. GISH analysis confirmed that 18 chromosomes came from trifoliate orange and the remaining 18 from Goutou sour orange, as with most symmetric somatic hybrid plants; moreover, chromosome translocations were also observed in one plant. AFLP analysis of 16 regenerates and their fusion parents indicated that all of the somatic hybrids except one were genetically uniform. Analysis of the somatic hybrid cytoplasmic genomes with universal primers rev...Continue Reading

References

Nov 11, 1995·Nucleic Acids Research·P VosM Kuiper
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A. BaroneL. Frusciante
Jul 8, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Guangmin XiaHuimin Chen
Jan 1, 1995·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y C Song, J P Gustafson

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