GK-1 improves the immune response induced by bone marrow dendritic cells loaded with MAGE-AX in mice with melanoma

Journal of Immunology Research
Gabriela Piñón-ZárateAndrés Eliú Castell-Rodríguez

Abstract

The aim of dendritic cell (DC) vaccination in cancer is to induce tumor-specific effector T cells that may reduce and control tumor mass. Immunostimulants that could drive a desired immune response are necessary to be found in order to generate a long lasting tumor immune response. GK-1 peptide, derived from Taenia crassiceps, induces not only increase in TNFα, IFNγ, and MCP-1 production in cocultures of DCs and T lymphocytes but also immunological protection against influenza virus. Moreover, the aim of this investigation is the use of GK-1 as a bone marrow DCs (BMDCs) immunostimulant targeted with MAGE antigen; thus, BMDC may be used as immunotherapy against murine melanoma. GK-1 induced in BMDCs a meaningful increment of CD86 and IL-12. In addition, the use of BMDCs TNFα/GK-1/MAGE-AX induced the highest survival and the smallest tumors in mice. Besides, the treatment helped to increase CD8 lymphocytes levels and to produce IFNγ in lymph nodes. Moreover, the histopathological analysis showed that BMDCs treated with GK-1/TNFα and loaded with MAGE-AX induced the apparition of more apoptotic and necrotic areas in tumors than in mice without treatment. These results highlight the properties of GK-1 as an immunostimulant of DCs an...Continue Reading

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Citations

Oct 2, 2019·Acta Parasitologica·Laila Gutiérrez-Kobeh, Arturo A Wilkins-Rodríguez
Jun 10, 2015·Theoretical Biology & Medical Modelling·E Castillo-MontielA E Castell-Rodríguez
Apr 30, 2016·International Journal of Environmental Research and Public Health·Pedro H Miranda-OsorioJuan C Díaz-Zagoya
Jan 30, 2016·Journal of Immunology Research·Gabriela Piñón-ZárateAndrés Eliú Castell-Rodríguez
Sep 16, 2017·Computational and Mathematical Methods in Medicine·J C Rangel-ReyesE Castillo-Montiel

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
flow cytometry

Software Mentioned

GraphPad Prism
Sigma Plot
Flow Jo
Motic Images Plus

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