PMID: 15348416Sep 7, 2004Paper

Gland cell cultures into 3D hyaluronan-based scaffolds

Journal of Materials Science. Materials in Medicine
Barbara ZavanG Abatangelo

Abstract

In this study we report a preliminary investigation of the feasibility of non-woven/sponge fabrics of a hyaluronan derived biomaterials (benzyl ester of HA (HYAFF-11 FAB, Abano Terme, Italy) for the in vitro culture of rat hepatocytes and rat beta cells. Cell growth on hyaluronan derived biomaterials were tested in the presence of complete medium and in the presence of ECM (extracellular matrix) secreted by fibroblasts previously cultured into the scaffold. Hepatocytes and beta cells were extracted from rat liver/pancreas and seeded either on the HYAFF-11 scaffold alone, or on HYAFF-11 scaffold containing ECM. Direct assay of cell proliferation was performed with MTT test. For morphological observations samples were stained with hematoxylin and eosin. The results obtained by MTT test showed that hepatocytes cultivated in both the above described conditions were able to proliferate up to 14 days and Langerhans islet up to 21 days. After this time, cells started to undergo apoptosis. The morphological analyses showed cell aggregation in three-dimensional structures promoted by the fibers of the biomaterial. Our results confirmed that HYAFF-11 meshes represent a suitable scaffold for hepatocyte adhesion/Langerhans islet organizati...Continue Reading

Citations

Feb 13, 2015·Plastic and Reconstructive Surgery. Global Open·Heidi DebelsWayne Morrison
May 24, 2007·Tissue Engineering·Zannatul Ferdous, K Jane Grande-Allen
Aug 20, 2010·Cell Transplantation·Jamal DaoudMaryam Tabrizian
Nov 7, 2012·Cell Transplantation·Yuan-Sheng LiTzyy-Wen Chiou
Sep 11, 2009·International Journal of Molecular Sciences·Vincenzo VindigniBarbara Zavan
Nov 30, 2016·International Journal of Molecular Sciences·Atul A ChaudhariShreekumar R Pillai
Mar 22, 2015·Cell and Tissue Research·Layasadat KhorsandiSadegh Saremy

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