PMID: 11931028Apr 5, 2002Paper

Glass capillary tube feeding: a method for infecting nymphal Ixodes scapularis (Acari: Ixodidae) with the lyme disease spirochete Borrelia burgdorferi

Journal of Medical Entomology
A BroadwaterSilva Aravinda M De

Abstract

We evaluated an artificial capillary feeding method to infect nymphal Ixodes scapularis (Say) ticks with Borrelia burgdoeferi, the causative agent of Lyme disease. Thirty to 70% of the nymphs were infected after feeding for 2.5 h from glass capillary tubes filled with a solution of spirochetes. Capillary infection was stable and persisted in the nymphs for at least 10 d after feeding. Capillary feeding also maintained natural vector competence patterns because I. scapularis ticks acquired infection unlike Dermacentor variablis (Say), which did not become infected. Capillary infected I. scapularis nymphs were capable of transmitting the infection to naive mice although not as efficiently as naturally infected nymphs. The capillary infection method is convenient and is a better alternative to syringe inoculation as a means of infecting animals with B. burgdorferi.

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Citations

Jan 22, 2013·Folia Microbiologica·Arunee AhantarigLibor Grubhoffer
Feb 3, 2005·Nature Reviews. Microbiology·Patricia A RosaPhilip E Stewart
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·J OhnishiA M de Silva
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Sep 29, 2005·Vector Borne and Zoonotic Diseases·Keith O StrotherAravinda De Silva
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Aug 4, 2005·Journal of Bacteriology·Keith O Strother, Aravinda de Silva
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Mar 5, 2011·Journal of Visualized Experiments : JoVE·Toru KariuUtpal Pal
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Nov 9, 2011·Comparative Immunology, Microbiology and Infectious Diseases·Sarah A BilleterBruno B Chomel
Aug 1, 2018·Vector Borne and Zoonotic Diseases·Jung Keun LeeAndrea S Varela-Stokes
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