Gli-1 siRNA induced apoptosis in Huh7 cells.

World Journal of Gastroenterology : WJG
Xi-Lin ChenXin-Hui Fu

Abstract

To investigate the effects of Gli-1 small interference RNA (siRNA) on Huh7 cells, and the change of Bcl-2 expression in Huh7 cells. Human hepatocellular carcinoma cells Huh7 were used. Cell viability was analyzed by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The expressions of Gli-1 and Bcl-2 family members were detected by RT-PCR and Western blot. Apoptosis was detected by Flow cytometry using propidium iodide, measured by Hoechst 33258 staining using Advanced Fluorescence Microscopy and caspase-3 enzymatic assay. Cell growth was analyzed after treatment with Gli-1 siRNA and 5-fluorouracil (5-Fu). Inhibition of Gli-1 mRNA in Huh7 cells through Gli-1 siRNA reduced cell viability. Gli-1 siRNA treatment also induced apoptosis by three criteria, increase in the sub-G1 cell cycle fraction, nuclear condensation, a morphologic change typical of apoptosis, and activation of caspase-3. Gli-1 siRNA was also able to down-regulate Bcl-2. However, Gli-1 siRNA resulted in no significant changes in Bcl-xl, Bax, Bad, and Bid. Furthermore, Gli-1 siRNA increased the cytotoxic effect of 5-Fu on Huh7 cell. Down-regulation of Bcl-2 plays an important role in apoptosis induced by Gli-1 siRNA in HCC cells. Combinati...Continue Reading

References

Oct 30, 1980·Nature·C Nüsslein-Volhard, E Wieschaus
Mar 13, 1999·The Journal of Biological Chemistry·P DaiS Ishii
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S H ShinL G Biesecker
Dec 4, 2001·Genes & Development·P W Ingham, A P McMahon
Feb 2, 2002·Nature Reviews. Neuroscience·Ariel Ruiz i AltabaNadia Dahmane
Jun 18, 2002·Nature Genetics·Michael D TaylorDavid Hogg
Aug 31, 2002·Science·David M BermanPhilip A Beachy
Nov 5, 2002·Genes & Development·James K ChenPhilip A Beachy
Apr 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juliet A WilliamsFrank Y Wang
Jun 19, 2003·Developmental Biology·Jun MotoyamaChi-chung Hui
Oct 2, 2003·Nature·Sarah P ThayerMatthias Hebrok
Oct 14, 2003·The Journal of Biological Chemistry·Rebecca L H BigelowTimothy J McDonnell
Oct 14, 2003·American Journal of Medical Genetics. Part a·M Michael Cohen
Jan 23, 2004·Nature Reviews. Cancer·Marina Pasca di Magliano, Matthias Hebrok
Jun 24, 2004·The Journal of Cell Biology·Vanessa S MarsdenAndreas Strasser
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Pilar SanchezAriel Ruiz i Altaba
Sep 14, 2004·Nature·Sunil S KarhadkarPhilip A Beachy
Jan 18, 2005·Mechanisms of Development·Pilar Sanchez, Ariel Ruiz i Altaba
Dec 13, 2005·Carcinogenesis·Jason K SicklickAnna Mae Diehl
Jan 7, 2006·Cancer Biology & Therapy·Mohini A PatilXin Chen
Feb 28, 2006·Carcinogenesis·Shuhong HuangJingwu Xie
Nov 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jennifer Sims-MourtadaK S Clifford Chao
Feb 6, 2007·Developmental Cell·Sarah Swerdlow, Clark W Distelhorst
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jennifer P MortonBrian C Lewis

❮ Previous
Next ❯

Citations

Aug 27, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yang XuRobert A Anders
Mar 19, 2009·World Journal of Gastroenterology : WJG·Jin-Bo WangTian-Xing Wu
Feb 6, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiao WangYandong Li
Jul 6, 2010·Biochemical and Biophysical Research Communications·Zhang PengjuJiang Anli
May 17, 2011·Journal of Neuropathology and Experimental Neurology·Pawel BuczkowiczCynthia Hawkins
Jun 10, 2008·Experimental and Molecular Pathology·Xinhui FuLianzhou Chen
Feb 22, 2011·Biotechnology and Bioengineering·Aliaksandr DruzJoseph Shiloach
Feb 15, 2015·Critical Reviews in Oncology/hematology·Kuo-Shyang JengChi-Juei Jeng
Oct 17, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Heng CaiDong Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis