Gli1+ Cells Couple with Type H Vessels and Are Required for Type H Vessel Formation.

Stem Cell Reports
Ji ChenCheng-Hu Hu

Abstract

Mesenchymal stem/stromal cells (MSCs) reside in the perivascular niche and modulate tissue/organ homeostasis; however, little is known about whether and how their localization and function are linked. Particularly, whether specific MSC subsets couple with and regulate specialized vessel subtypes is unclear. Here, we show that Gli1+ cells, which are a subpopulation of MSCs couple with and regulate a specialized form of vasculature. The specific capillaries, i.e., CD31hiEMCNhi type H vessels, are the preferable vascular subtype which Gli1+ cells are adjacent to in bone. Gli1+ cells are further identified to be phenotypically coupled with type H endothelium during bone growth and defect healing. Importantly, Gli1+ cell ablation inhibits type H vessel formation associated with suppressed bone generation and regeneration. Mechanistically, Gli1+ cells initiate angiogenesis through Gli and HIF-1α signaling. These findings suggest a morphological and functional framework of Gli1+ cells modulating coupled type H vasculature for tissue homeostasis and regenerative repair.

References

Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Songtao Shi, Stan Gronthos
Nov 4, 2005·Genesis : the Journal of Genetics and Development·Anna IvanovaJuan Pedro Martinez-Barbera
Feb 6, 2007·Developmental Cell·Kristy Red-HorseNapoleone Ferrara
Jun 6, 2007·The Journal of Clinical Investigation·Ying WangThomas L Clemens
Sep 9, 2008·Journal of Cellular and Molecular Medicine·Maarten F BijlsmaC Arnold Spek
Sep 13, 2008·Cell Stem Cell·Mihaela CrisanBruno Péault
Dec 17, 2008·Developmental Cell·Jin Jiang, Chi-Chung Hui
Aug 13, 2010·Nature·Simón Méndez-FerrerPaul S Frenette
Mar 22, 2014·Nature·Saravana K RamasamyRalf H Adams
Dec 4, 2014·Cell Stem Cell·Rafael KramannBenjamin D Humphreys
Dec 17, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jianjun LeiXuqi Li
Mar 10, 2015·Cell Stem Cell·Youmna Kfoury, David T Scadden
Mar 24, 2015·Nature Cell Biology·Hu ZhaoYang Chai
Aug 19, 2015·The Journal of Clinical Investigation·Nicholas A ManieriThaddeus S Stappenbeck
Oct 30, 2015·Nature Protocols·Anjali P KusumbeRalf H Adams
Jan 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shailesh AgarwalBenjamin Levi
Apr 14, 2016·Nature·Anjali P KusumbeRalf H Adams
Apr 23, 2016·Stem Cells Translational Medicine·Christoph WallnerBjörn Behr
Jul 17, 2016·Journal of Dental Research·N ChenF Jin
Aug 4, 2016·Development·Kishor K Sivaraj, Ralf H Adams
Dec 15, 2016·Nature Communications·Bin HuangXiaochun Bai
Jan 24, 2017·Cell Stem Cell·Nuno Guimarães-CamboaSylvia M Evans
Feb 28, 2017·Scientific Reports·Cheng-Hu HuYan Jin
Dec 13, 2017·Nature Communications·Yu ShiFanxin Long
Feb 2, 2018·Science Translational Medicine·Dario R Lemos, Jeremy S Duffield
May 23, 2018·Nature Medicine·Ren XuMatthew B Greenblatt
Mar 29, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Robin CaireMarie-Hélène Lafage-Proust
Feb 16, 2020·Cell·Joanna KaluckaPeter Carmeliet

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Citations

Nov 25, 2020·Journal of Hematology & Oncology·Lei ZhangJiwang Zhang
Feb 4, 2021·Journal of Cellular Physiology·Dian JingJun Wang
Jun 5, 2021·Journal of Extracellular Vesicles·Chenxi ZhengYan Jin
Sep 5, 2021·Nature Reviews. Rheumatology·Jan Tuckermann, Ralf H Adams
Oct 9, 2021·Bone·Tareq Anani, Alesha B Castillo
Nov 6, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ziyan WangChao Liu

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Methods Mentioned

BETA
PCR
transgenics
flow cytometry

Software Mentioned

GraphPad Prism
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FV10
ASW Viewer
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