Glia cell line-derived neurotrophic factor regulates the distribution of acetylcholine receptors in mouse primary skeletal muscle cells

Neuroscience
L-X Yang, P G Nelson

Abstract

It was recently reported that glia cell line-derived neurotrophic factor (GDNF) facilitates presynaptic axonal growth and neurotransmitter release at neuromuscular synapses. Little is known, however, whether GDNF can also act on the postsynaptic apparatus and its underlying mechanisms. Using biochemical cold blocking of existing membrane acetylcholine receptors (AchRs) and biotinylation of newly inserted receptors we demonstrate that GDNF increases the insertion of AChRs into the surface membrane of mouse primary cultured muscle cells and that this does not require protein synthesis. Quantitative data from double-label imaging indicate that GDNF induces a quick and substantial increase in AchR insertion as well as lateral movement into AchR aggregates, relative to a weak effect on reducing the loss of receptors from pre-existing AchR aggregates, which in contrast to the effect of PMA. These effects occur in both innervated and un-innervated muscles, and GDNF affects nerve-muscle co-cultures more than it affects muscle-only cultures. Neurturin, another member of GDNF-family ligands has similar effects on AchRs as GDNF but the unrelated growth factor, EGF does not. Studies on protein phosphorylation and specific inhibitors of cel...Continue Reading

References

Jul 1, 1985·The Journal of Cell Biology·F RiegerG M Edelman
Mar 1, 1986·The Journal of Cell Biology·J CovaultJ R Sanes
Jan 1, 1994·Journal of Physiology, Paris·L A RaymondR L Huganir
Jul 4, 1996·Nature·J J TreanorA Rosenthal
Jan 31, 1998·The Journal of Biological Chemistry·S JingG M Fox
Feb 19, 1998·Oncogene·C NozakiM Takahashi
Apr 16, 1998·Biochemical and Biophysical Research Communications·S NomotoK Kiuchi
Mar 11, 1999·Current Opinion in Neurobiology·E M Schuman
Apr 15, 1999·Annual Review of Neuroscience·A K McAllisterD C Lo
Apr 15, 1999·Annual Review of Neuroscience·J R Sanes, J W Lichtman
Apr 28, 1999·Advances in Second Messenger and Phosphoprotein Research·S L SwopeR L Huganir
Feb 19, 2000·Current Opinion in Neurobiology·R H BalohJ Milbrandt

❮ Previous
Next ❯

Citations

Sep 18, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masatoshi SuzukiClive N Svendsen
Aug 24, 2012·Journal of Neurophysiology·Pedro AlvarezJon D Levine
Oct 1, 2011·Journal of Biomedicine & Biotechnology·Kunihiro Sakuma, Akihiko Yamaguchi
Feb 1, 2011·Pflügers Archiv : European journal of physiology·Tian LiChristopher S von Bartheld
Aug 29, 2014·Nature Reviews. Neuroscience·Darran Yates
Oct 1, 2008·Progress in Neurobiology·Ana SaavedraEmília P Duarte
Aug 19, 2008·Respiratory Physiology & Neurobiology·Carlos B Mantilla, Gary C Sieck
Jul 1, 2010·Biotechnology and Bioengineering·Matthew D WoodShelly E Sakiyama-Elbert
Oct 27, 2010·Muscle & Nerve·Gizelda T B CasellaChristine K Thomas
Oct 22, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Antos ShakhbazauRajiv Midha
Jan 1, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Serena StangaPascal Kienlen-Campard
Sep 8, 2017·Frontiers in Molecular Neuroscience·Daniel CortésIván Velasco

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.