Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

The Journal of Biological Chemistry
Wulin AerbajinaiGriffin P Rodgers

Abstract

Monocyte migration requires the dynamic redistribution of integrins through a regulated endo-exocytosis cycle, but the complex molecular mechanisms underlying this process have not been fully elucidated. Glia maturation factor-γ (GMFG), a novel regulator of the Arp2/3 complex, has been shown to regulate directional migration of neutrophils and T-lymphocytes. In this study, we explored the important role of GMFG in monocyte chemotaxis, adhesion, and β1-integrin turnover. We found that knockdown of GMFG in monocytes resulted in impaired chemotactic migration toward formyl-Met-Leu-Phe (fMLP) and stromal cell-derived factor 1α (SDF-1α) as well as decreased α5β1-integrin-mediated chemoattractant-stimulated adhesion. These GMFG knockdown impaired effects could be reversed by cotransfection of GFP-tagged full-length GMFG. GMFG knockdown cells reduced the cell surface and total protein levels of α5β1-integrin and increased its degradation. Importantly, we demonstrate that GMFG mediates the ubiquitination of β1-integrin through knockdown or overexpression of GMFG. Moreover, GMFG knockdown retarded the efficient recycling of β1-integrin back to the plasma membrane following normal endocytosis of α5β1-integrin, suggesting that the involve...Continue Reading

References

Nov 3, 1994·Nature·J E Rothman
Nov 24, 1999·Nature Cell Biology·M K Bennett
May 3, 2000·The Journal of Cell Biology·I Mellman
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y A Chen, R H Scheller
Feb 26, 2003·Cell·Reinhard JahnThomas C Südhof
Mar 21, 2003·Current Biology : CB·Fanny S ChangKendall J Blumer
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Mar 9, 2005·The Journal of Cell Biology·Eijiro YamadaMasatomo Mori
Feb 1, 2006·Traffic·Patrick T Caswell, Jim C Norman
Aug 15, 2006·Current Opinion in Cell Biology·Matthew C JonesJim C Norman
Sep 9, 2006·Journal of Cell Science·Teijo Pellinen, Johanna Ivaska
Mar 6, 2007·Biochemical and Biophysical Research Communications·Shuichi OkadaMasatomo Mori
Aug 25, 2007·Nature Reviews. Immunology·Klaus LeySussan Nourshargh
Jan 2, 2009·Journal of Cell Science·Maria Rosaria CeraElisabetta Dejana
Mar 18, 2009·Developmental Cell·Etienne MorelJean Gruenberg
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Barth D Grant, Julie G Donaldson
Nov 12, 2009·Nature Reviews. Molecular Cell Biology·Patrick T CaswellJim C Norman
Nov 20, 2009·Developmental Cell·Emmanuel DeriveryAlexis Gautreau
Dec 8, 2009·Science·Thomas D Pollard, John A Cooper
Dec 17, 2009·Biochemical and Biophysical Research Communications·Takayuki KawaguchiMasato Kasuga
Feb 4, 2010·Cardiovascular Research·Connie H Y WongPaul Kubes
Mar 24, 2010·Nature Reviews. Molecular Cell Biology·Sanford J ShattilMark H Ginsberg
Jun 15, 2010·Current Opinion in Cell Biology·Victor W Hsu, Rytis Prekeris
Nov 26, 2010·Current Opinion in Cell Biology·Elif Nur Firat-Karalar, Matthew D Welch
Dec 15, 2010·Developmental Cell·Lunhua LiuCarole A Parent
Apr 9, 2011·Current Opinion in Cell Biology·Harald W Platta, Harald Stenmark
Jun 10, 2011·Journal of Leukocyte Biology·Wulin AerbajinaiGriffin P Rodgers
Sep 2, 2011·The Journal of Biological Chemistry·Ajit TiwariAmit Choudhury
Sep 20, 2011·Current Opinion in Cell Biology·Coert MargadantArnoud Sonnenberg
Jan 10, 2012·Traffic·Antti ArjonenJohanna Ivaska
Mar 27, 2012·Communicative & Integrative Biology·Kenji TanabeKohji Takei
Apr 12, 2012·The Journal of Cell Biology·Florian SteinbergPeter J Cullen
Apr 19, 2012·BMC Immunology·Dustin N D Lippert, John A Wilkins
May 9, 2012·Nature Cell Biology·Ralph Thomas BöttcherReinhard Fässler

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Citations

Feb 28, 2019·American Journal of Respiratory Cell and Molecular Biology·Brennan D GerlachDale D Tang
May 11, 2019·Cellular and Molecular Life Sciences : CMLS·Sandra Chánez-ParedesMichael Schnoor
May 22, 2018·Trends in Cell Biology·Bruce L GoodeJulian A Eskin
Jul 1, 2021·Molecular and Cellular Biochemistry·Lediana I MiguelNicola Conran

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