Glial cell activity is maintained during prolonged inflammatory challenge in rats.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
B C BorgesL L K Elias

Abstract

We evaluated the expression of glial fibrillary acidic protein (GFAP), glutamine synthetase (GS), ionized calcium binding adaptor protein-1 (Iba-1), and ferritin in rats after single or repeated lipopolysaccharide (LPS) treatment, which is known to induce endotoxin tolerance and glial activation. Male Wistar rats (200-250 g) received ip injections of LPS (100 µg/kg) or saline for 6 days: 6 saline (N = 5), 5 saline + 1 LPS (N = 6) and 6 LPS (N = 6). After the sixth injection, the rats were perfused and the brains were collected for immunohistochemistry. After a single LPS dose, the number of GFAP-positive cells increased in the hypothalamic arcuate nucleus (ARC; 1 LPS: 35.6 ± 1.4 vs control: 23.1 ± 2.5) and hippocampus (1 LPS: 165.0 ± 3.0 vs control: 137.5 ± 2.5), and interestingly, 6 LPS injections further increased GFAP expression in these regions (ARC = 52.5 ± 4.3; hippocampus = 182.2 ± 4.1). We found a higher GS expression only in the hippocampus of the 6 LPS injections group (56.6 ± 0.8 vs 46.7 ± 1.9). Ferritin-positive cells increased similarly in the hippocampus of rats treated with a single (49.2 ± 1.7 vs 28.1 ± 1.9) or repeated (47.6 ± 1.1 vs 28.1 ± 1.9) LPS dose. Single LPS enhanced Iba-1 in the paraventricular nucleus...Continue Reading

References

Jan 1, 1991·Glia·M E HattenC A Mason
Nov 1, 1989·Cognition·C W Cotman, G S Lynch
Oct 1, 1988·Journal of Neuroscience Research·E HetierA Prochiantz
Jan 3, 1994·Neuroscience Letters·A Derouiche, T G Ohm
Jul 1, 1994·Brain Pathology·L F Eng, R S Ghirnikar
Feb 7, 2001·Science·E M UllianB A Barres
Oct 12, 2001·Glia·Y Dong, E N Benveniste
Mar 2, 2005·Best Practice & Research. Clinical Haematology·Günter Weiss
Apr 27, 2007·American Journal of Physiology. Cell Physiology·J FisherJ R Connor
Nov 6, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·J Catharina DuvigneauAndrey V Kozlov
Jun 10, 2008·Brain Research·Alfonso Romero-SandovalJoyce A Deleo
Feb 24, 2011·American Journal of Physiology. Endocrinology and Metabolism·Beatriz C BorgesLucila L K Elias

❮ Previous
Next ❯

Citations

Nov 2, 2013·Environmental Toxicology and Pharmacology·Rajapaksha Gedara Prasad Tharanga JayasooriyaGi-Young Kim
Nov 5, 2015·Journal of Neuroimmunology·Christina W FischerGregers Wegener
Jul 9, 2016·Molecular and Cellular Endocrinology·Beatriz C BorgesLucila L K Elias
Oct 30, 2014·American Journal of Physiology. Endocrinology and Metabolism·Beatriz de Carvalho BorgesLucila L K Elias
Nov 29, 2020·Glia·Sofia Pereira das NevesFernanda Marques

❮ Previous
Next ❯

Software Mentioned

Image J

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.