Glial cell-derived neurotrophic factor enhances synaptic communication and 5-hydroxytryptamine 3a receptor expression in enteric neurons

Gastroenterology
Fanning ZengMark S Nash

Abstract

Glial cell-derived neurotrophic factor (GDNF) is essential for the development of the enteric nervous system during embryogenesis. We have observed the presence of Gdnf transcripts in the gastrointestinal tract of adult mice, and its early up-regulation after inflammation. We therefore investigated the effects of GDNF on enteric neuronal function in vitro. Primary neuronal cultures were established from isolated myenteric plexi, and characterized by immunostaining and Ca(2+) imaging. Gene expression of several ion channels was analyzed by quantitative polymerase chain reaction (PCR) and the electrophysiologic properties of the neurons were studied by patch clamp. GDNF enhanced synaptogenesis and intercellular communication in primary myenteric neuronal cultures. Expression profiling revealed that GDNF exposure results in an up-regulation of Htr3a expression in the cultures and a similar increase was observed in inflamed colonic tissue where Gdnf expression was also increased. The increased Htr3a expression was accompanied by a functional increase in the response of neurons to acute challenge with 5-hydroxytryptamine (5-HT). GDNF treatment also caused inhibition of delayed rectifying voltage-gated potassium (Kv) currents, which ...Continue Reading

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Citations

Nov 8, 2012·PloS One·Fanning ZengMark S Nash
Dec 23, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Gabrio BassottiCorrado R Asteria
Nov 23, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D M RodriguesM G Blennerhassett
Jan 26, 2011·Glia·Gabrio Bassotti, Vincenzo Villanacci
Nov 5, 2011·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Robert P WatsonMark S Nash
Jul 7, 2017·PloS One·Tomokazu HataNobuyuki Sudo
Feb 15, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kenjiro MatsumotoSyunji Horie
Nov 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kenjiro Matsumoto
May 8, 2019·Acta Physiologica·Michael SchemannPaul Enck
May 23, 2013·Digestive Diseases and Sciences·Kenjiro MatsumotoSyunji Horie
Sep 8, 2017·Frontiers in Molecular Neuroscience·Daniel CortésIván Velasco
Nov 18, 2020·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Fenghua ChenGregers Wegener
Jun 26, 2021·Journal of Cellular Physiology·Sangeeta BhallamudiY S Prakash
Dec 1, 2021·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·HuiLing ChenDeKui Zhang

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