PMID: 10963359Aug 30, 2000

Glial cell line-derived neurotrophic factor-like immunoreactivity in the cerebella of normal subjects and patients with multiple system atrophy

Acta Neuropathologica
Y KawamotoI Akiguchi

Abstract

Glial cell line-derived neurotrophic factor (GDNF) has a trophic effect on various types of neurons, including cerebellar Purkinje cells. To investigate the role of GDNF in the human cerebellum, we examined the cerebella of eight control cases and eight patients with multiple system atrophy (MSA) immunohistochemically using a polyclonal anti-GDNF antibody. The antibody recognized a single band of approximately 34 kDa on Western blot analysis of human cerebellar homogenates. In the cerebella from normal subjects, the neuronal somata and dendrites of the Purkinje cells were immunostained intensely, as were some axons, including torpedoes, immunolabeled in the granular layer. Many axons and a few oligodendrocytes were also immunopositive in the white matter, and weak immunoreactivity was detected in the granule cells and neurons in the cerebellar nuclei. In the cerebella from patients with MSA, the general immunostaining pattern was similar to that observed in the normal subjects. Most of the remaining Purkinje cells showed strong immunoreactivity, and abundant GDNF-positive granular structures or dense arborizations of GDNF-positive dendrites were found in some areas of the molecular layer. These data suggest that GDNF may be mai...Continue Reading

References

Jan 25, 2005·Acta Neuropathologica·Gregor K Wenning, Kurt A Jellinger
Oct 16, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·H Koo, B H Choi
Oct 10, 2014·Movement Disorders : Official Journal of the Movement Disorder Society·Kurt A Jellinger
Jun 15, 2014·Clinical Autonomic Research : Official Journal of the Clinical Autonomic Research Society·Jose-Alberto Palma, Horacio Kaufmann
Jul 12, 2002·Journal of Neuroscience Research·Yangzhou Du, Cheryl F Dreyfus

Related Concepts

Congenital Structural Myopathy
Immunoreactivity
Cerebellar Nuclei
Neurons
Purkinje Cells
Stratum Granulosum
GDNF gene
Immunocytochemistry
Neurite Outgrowth Factors
Western Blot

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